Hsu Shih-Chung, Ou Chien-Chih, Chuang Tzu-Chao, Li Jhy-Wei, Lee Yi-Jen, Wang Vinchi, Liu Jah-Yao, Chen Chin-Shiang, Lin Song-Chow, Kao Ming-Ching
Graduate Institute of Medical Sciences, Taipei Medical University, Taiwan, ROC.
Cancer Lett. 2009 Aug 18;281(1):108-16. doi: 10.1016/j.canlet.2009.02.032. Epub 2009 Mar 29.
We examined the anti-angiogenic effects of Ganoderma tsugae methanol extract (GTME) on human epidermoid carcinoma A-431 cells. Our data indicate that GTME inhibits the expression of epidermal growth factor receptor (EGFR) and vascular endothelial growth factor (VEGF) in vitro and in vivo, and also inhibits the capillary tube formation of human umbilical vein endothelial cells (HUVECs). We also show that the suppression of VEGF expression by GTME can be restored by treatment with EGF. These results suggest that GTME inhibits VEGF expression via the suppression of EGFR expression, resulting in the downregulation of VEGF secretion from epidermoid carcinoma A-431 cells. These findings reveal a novel role for G. tsugae in inhibiting EGFR and VEGF expression, which are important for tumor angiogenesis and growth. Thus, GTME may provide a potential therapeutic approach for anti-tumor treatment.
我们研究了松杉灵芝甲醇提取物（GTME）对人表皮样癌A - 431细胞的抗血管生成作用。我们的数据表明，GTME在体外和体内均抑制表皮生长因子受体（EGFR）和血管内皮生长因子（VEGF）的表达，并且还抑制人脐静脉内皮细胞（HUVECs）的毛细血管管形成。我们还表明，用表皮生长因子（EGF）处理可恢复GTME对VEGF表达的抑制作用。这些结果表明，GTME通过抑制EGFR表达来抑制VEGF表达，从而导致表皮样癌A - 431细胞中VEGF分泌的下调。这些发现揭示了松杉灵芝在抑制EGFR和VEGF表达方面的新作用，而这对肿瘤血管生成和生长很重要。因此，GTME可能为抗肿瘤治疗提供一种潜在的治疗方法。
