Yan Zhun, Berbenetz Nicolas M, Giaever Guri, Nislow Corey
Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada.
Genetics. 2009 Jun;182(2):623-6. doi: 10.1534/genetics.109.103036. Epub 2009 Mar 30.
Modulating gene dose is an effective way to alter protein levels and modify phenotypes to understand gene function. In addition, combining gene-dose alleles with chemical perturbation can provide insight into drug-gene interactions. Here, we present a strategy that combines diverse loss-of-function alleles to systematically modulate gene dose in Saccharomyces cerevisiae. The generated gene dosage allele set expands the genetic toolkit for uncovering novel phenotypes.
调节基因剂量是改变蛋白质水平和修饰表型以了解基因功能的有效方法。此外，将基因剂量等位基因与化学扰动相结合可以深入了解药物 - 基因相互作用。在这里，我们提出了一种策略，该策略结合了多种功能丧失等位基因，以系统地调节酿酒酵母中的基因剂量。所产生的基因剂量等位基因集扩展了用于揭示新表型的遗传工具包。
