Young L T, Li P P, Kish S J, Siu K P, Warsh J J
Department of Psychiatry, University of Toronto, Clarke Institute of Psychiatry, Toronto, Canada.
Brain Res. 1991 Jul 12;553(2):323-6. doi: 10.1016/0006-8993(91)90843-k.
We examined the relative abundance of G-protein subunits in postmortem brain obtained from 7 patients with bipolar affective disorder (BAD) compared with 7 age- and sex-matched controls. G-protein subunit immunoreactivities were determined in membranes prepared from postmortem prefrontal cortex using SDS-PAGE and immunoblotting with specific polyclonal antisera against selected G-protein subunits: Gsa, Gi(1&2) alpha, Go alpha and G beta(1&2). Of these G-protein subunits, only Gs alpha immunoreactivity was found to be significantly elevated in frontal (+ 34%), and occipital (+ 80%) cortex (P less than 0.05) in BAD compared with control subjects. Smaller increments (+ 22%) in cerebellar Gs alpha immunoreactivity were also found but were not statistically significant. On the basis that increased Gs alpha immunoreactivity may reflect enhanced functional responsiveness of the receptor-effector units to which this coupling protein is integral, the present findings suggest that disturbances in Gs-mediated signal transduction may be involved in the pathophysiology of BAD.
我们检测了7例双相情感障碍（BAD）患者死后大脑中G蛋白亚基的相对丰度，并与7例年龄和性别匹配的对照者进行比较。使用SDS-PAGE和针对选定G蛋白亚基（Gsα、Gi(1&2)α、Goα和Gβ(1&2)）的特异性多克隆抗血清进行免疫印迹，测定死后前额叶皮质制备的膜中的G蛋白亚基免疫反应性。在这些G蛋白亚基中，与对照受试者相比，仅发现BAD患者额叶（+34%）和枕叶（+80%）皮质中的Gsα免疫反应性显著升高（P<0.05）。小脑Gsα免疫反应性也有较小幅度的增加（+22%），但无统计学意义。基于Gsα免疫反应性增加可能反映了该偶联蛋白所参与的受体-效应器单位功能反应性增强，目前的研究结果表明，Gs介导的信号转导紊乱可能参与了BAD的病理生理过程。
