Young L T, Asghari V, Li P P, Kish S J, Fahnestock M, Warsh J J
Department of Psychiatry, McMaster University, Hamilton, Ont. Canada.
Brain Res Mol Brain Res. 1996 Nov;42(1):45-50. doi: 10.1016/s0169-328x(96)00112-x.
Increased alpha-subunit (alpha s) levels of both the 45- and 52-kDa isoforms of the stimulatory guanine nucleotide binding protein (G-protein), have been found in postmortem brain and mononuclear leukocytes from patients with bipolar disorder (BD). The pathophysiological mechanism responsible for increased alpha s protein levels is unknown, however, it may involve increased expression of the gene encoding this protein. To assess this possibility, alpha s mRNA levels were determined by RT-PCR in postmortem brain from 10 subjects with an antemortem diagnosis of BD and age- and sex-matched control subjects in whom we had previously reported increased alpha s protein levels. There were no significant differences in alpha s mRNA levels in frontal, temporal, or occipital cortex between BD and control subjects. Cerebral cortex alpha s mRNA levels did not correlate with age or postmortem interval. These findings do not support the notion that higher alpha s levels found in BD postmortem brain are a result of increased gene expression.
在双相情感障碍（BD）患者的尸检脑和单核白细胞中，已发现刺激性鸟嘌呤核苷酸结合蛋白（G蛋白）的45 kDa和52 kDa亚型的α亚基（αs）水平升高。然而，导致αs蛋白水平升高的病理生理机制尚不清楚，它可能涉及编码该蛋白的基因表达增加。为了评估这种可能性，我们通过逆转录聚合酶链反应（RT-PCR）测定了10名生前诊断为BD的受试者以及年龄和性别匹配的对照受试者尸检脑中的αs mRNA水平，我们之前报告过这些对照受试者的αs蛋白水平升高。BD患者与对照受试者在额叶、颞叶或枕叶皮质的αs mRNA水平没有显著差异。大脑皮质αs mRNA水平与年龄或死后间隔无关。这些发现不支持BD患者尸检脑中较高的αs水平是基因表达增加所致的观点。
