Heymann-Mönnikes I, Taché Y, Trauner M, Weiner H, Garrick T
Department Psychiatry and Research, West Los Angeles Veterans Administration Medical Center, CA.
Brain Res. 1991 Jul 19;554(1-2):139-44. doi: 10.1016/0006-8993(91)90181-t.
The effect of CRF microinjected into the dorsal vagal complex (DVC) on centrally-stimulated gastric contractility was investigated in fasted, urethane-anesthetized rats. Miniature strain gauge force transducers were acutely implanted on the corpus of the stomach and contractility was analyzed by computer. Microinjection of the stable thyrotropin-releasing hormone (TRH) analog, RX 77368, (26 pmol) into the DVC induced a 12.2-fold stimulation of gastric contractility within 30 min. Corticotropin-releasing factor (CRF) (63-210 pmol) microinjected into the DVC concomitantly with RX 77368 (26 pmol) induced a dose-related inhibition of stimulated gastric contractility. Neither CRF alone (210 pmol) nor vehicle modified basal gastric contractility. Microinjection of kainic acid (141 pmol) into the raphe pallidus nucleus induced a 3.6-fold stimulation of gastric contractility after 45 min. This stimulation was suppressed by bilateral microinjection of CRF (105 pmol/site) into the DVC. These results demonstrate that CRF acts in the DVC to inhibit centrally-stimulated gastric contractility and suggest that TRH and CRF may interact in the DVC to regulate gastric motor function.
在禁食、氨基甲酸乙酯麻醉的大鼠中，研究了向迷走神经背核（DVC）微量注射促肾上腺皮质激素释放因子（CRF）对中枢刺激引起的胃收缩性的影响。将微型应变片式力传感器急性植入胃体，并通过计算机分析收缩性。向DVC微量注射稳定的促甲状腺激素释放激素（TRH）类似物RX 77368（26 pmol），可在30分钟内使胃收缩性受到12.2倍的刺激。与RX 77368（26 pmol）同时向DVC微量注射促肾上腺皮质激素释放因子（CRF）（63 - 210 pmol），可引起与剂量相关的对刺激胃收缩性的抑制。单独的CRF（210 pmol）或溶剂均未改变基础胃收缩性。向中缝苍白核微量注射海藻酸（141 pmol），45分钟后可使胃收缩性受到3.6倍的刺激。向DVC双侧微量注射CRF（105 pmol/部位）可抑制这种刺激。这些结果表明，CRF在DVC中发挥作用以抑制中枢刺激引起的胃收缩性，并提示TRH和CRF可能在DVC中相互作用以调节胃运动功能。
