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Processing of X-ray diffraction data collected in oscillation mode.振荡模式下收集的X射线衍射数据的处理。
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Insights into RNA/DNA hybrid recognition and processing by RNase H from the crystal structure of a non-specific enzyme-dsDNA complex.通过非特异性酶-dsDNA复合物的晶体结构深入了解RNase H对RNA/DNA杂交体的识别与加工
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SHELXL: high-resolution refinement.SHELXL：高分辨率精修。
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Selenium modification of nucleic acids: preparation of oligonucleotides with incorporated 2'-SeMe-uridine for crystallographic phasing of nucleic acid structures.核酸的硒修饰：用于核酸结构晶体学相位分析的掺入2'-硒甲基尿苷的寡核苷酸的制备。
Nat Protoc. 2007;2(3):647-51. doi: 10.1038/nprot.2007.75.
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2'-Fluoroarabino- and arabinonucleic acid show different conformations, resulting in deviating RNA affinities and processing of their heteroduplexes with RNA by RNase H.2'-氟阿拉伯糖核酸和阿拉伯糖核酸呈现出不同的构象，导致它们与RNA的亲和力不同，以及核糖核酸酶H对其与RNA形成的异源双链体的加工情况也不同。
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Changes in DNA bending induced by restricting nucleotide ring pucker studied by weak alignment NMR spectroscopy.通过弱排列核磁共振光谱研究限制核苷酸环皱折引起的DNA弯曲变化。
Proc Natl Acad Sci U S A. 2005 Jan 4;102(1):24-8. doi: 10.1073/pnas.0408498102. Epub 2004 Dec 23.
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RNA interference and chemically modified small interfering RNAs.RNA干扰与化学修饰的小干扰RNA
Curr Opin Chem Biol. 2004 Dec;8(6):570-9. doi: 10.1016/j.cbpa.2004.10.007.
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PROSIT: pseudo-rotational online service and interactive tool, applied to a conformational survey of nucleosides and nucleotides.PROSIT：伪旋转在线服务和交互式工具，应用于核苷和核苷酸的构象研究。
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Structural requirements at the catalytic site of the heteroduplex substrate for human RNase H1 catalysis.人源核糖核酸酶H1催化作用中异源双链底物催化位点的结构要求。
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在高分辨率下观察到的2'-硫代甲基修饰DNA结构中的构象转变。

A conformational transition in the structure of a 2'-thiomethyl-modified DNA visualized at high resolution.

作者信息

Pallan Pradeep S, Prakash Thazha P, Li Feng, Eoff Robert L, Manoharan Muthiah, Egli Martin

机构信息

Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.

出版信息

Chem Commun (Camb). 2009 Apr 21(15):2017-9. doi: 10.1039/b822781k. Epub 2009 Feb 26.

DOI:10.1039/b822781k
PMID:19333476
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2909737/
Abstract

Crystal structures of A-form and B-form DNA duplexes containing 2'-S-methyl-uridines reveal that the modified residues adopt a RNA-like C3'-endo pucker, illustrating that the replacement of electronegative oxygen at the 2'-carbon of RNA by sulfur does not appear to fundamentally alter the conformational preference of the sugar in the oligonucleotide context and sterics trump stereoelectronics.

摘要

含有2'-硫代甲基尿苷的A-型和B-型DNA双链体的晶体结构表明，修饰后的残基呈现出类似RNA的C3'-内向型构象，这说明在寡核苷酸环境中，RNA 2'-碳上的电负性氧被硫取代似乎并未从根本上改变糖的构象偏好，且空间效应胜过立体电子效应。