Jasonni V M, Melega C, Possati G, Preti M S, Crea P, Lodi S, Flamigni C
Acta Eur Fertil. 1976 Sep;7(3):187-213.
Plasma 17alpha OH progesterone was assayed radioimmunologically, using an anti-17-OH-progesterone BSA antiserum as specific binding antigen. The method consisted in extracting steroids with ethyl ether from 1 ml plasm (to which 17alpha OH progesterone-1,2-3H had been added for the assessment of losses); the extract was then submitted to chromatography on a Sephadex LH-20 column, antiserum was added and the free steroid was separated from the bound form by Charcoal-dextran. Average recovery of 17alpha OH progesterone-1,2-3H was 75+/-5%. Minimum sensitivity of the method oscillated about 7-10 pg. MIF (Method Interfering Factors) were evaluated. The method was used for the assay of 17alpha OH progesterone plasma levels during normal menstrual cycles, in women undergoing ovarian stimulation with HMG and HCG in the follicular and luteal phase respectively. Finally, changes of plasma 17alpha OH progesterone levels were assessed during adrenal stimulation and suppression in various stages of the menstrual cycle.
采用抗17-OH-孕酮牛血清白蛋白抗血清作为特异性结合抗原，放射免疫法测定血浆17α-羟孕酮。该方法包括用乙醚从1ml血浆中提取类固醇（已加入17α-羟孕酮-1,2,3H以评估损失）；提取物随后在葡聚糖凝胶LH-20柱上进行色谱分析，加入抗血清，并用活性炭-葡聚糖将游离类固醇与结合形式分离。17α-羟孕酮-1,2,3H的平均回收率为75±5%。该方法的最低灵敏度在7-10pg左右波动。评估了方法干扰因素（MIF）。该方法用于测定正常月经周期期间、分别在卵泡期和黄体期接受HMG和HCG卵巢刺激的女性血浆17α-羟孕酮水平。最后，评估了月经周期不同阶段肾上腺刺激和抑制期间血浆17α-羟孕酮水平的变化。
