Zhang Xianqing, Wu Daocheng, Mu Shuie, Xia Aijunn, Huang Xiaofeng, Wang Xiaolan
The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Xi'an Jiaotong University, Xi'an 710038, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2009 Feb;26(1):144-7, 152.
This study sought to shed light on the killing effect of peripheral blood mononuclear cells (PBMCs) irradiated by gamma ray at a dose of 1 Gy on cultured human gastric tumor cell line MKN-28. The radiation dose rate of 17 Gy/min was used. The groups in the experiment were MKN-28 cell control group, PBMCs control group, MKN-28 tumor cells with irradiated or non-irradiated PBMCs co-cultured groups. Radiation dosage was one Gray, acridine orange/ethidium bromide (AO/EB) staining was used for observation of the killing effects of PBMCs on tumor cells in different period. Cells were harvested 240 h later and observed by transmission electron microscopy. The result showed the living period of irradiated PBMCs was shorter than that of non-irradiated PBMCs. In the irradiated and non-irradiated groups,a few PBMCs were still alive after being cultured for 240 h, but the cell volume was larger than that of lymphocytes. These cells were identified as monocytes (95%) or DCs (5%) by transmission electron microscopy. The co-culture of irradiated PBMCs and MKN-28 cells showed that tumor cells were eliminated after 96 h. As compared with the non-irradiated goup, the irradiated PBMCs had more potent ability for killing tumor. The results demonstrate that 1 Gy gamma irridiation can improve the killing effect of PBMCs on the tumor cells, and that 1 Gy gamma irritation can also induce shorter living period of lymphocytes in PBMCs cultured in vitro, but such irritation has little effect on the living period of monocytes and DCs in PBMCs.
本研究旨在探讨剂量为1 Gy的γ射线辐照外周血单个核细胞（PBMC）对培养的人胃癌细胞系MKN - 28的杀伤作用。使用的辐射剂量率为17 Gy/min。实验分组为MKN - 28细胞对照组、PBMC对照组、MKN - 28肿瘤细胞与经辐照或未辐照的PBMC共培养组。辐射剂量为1 Gy，采用吖啶橙/溴化乙锭（AO/EB）染色观察PBMC在不同时期对肿瘤细胞的杀伤作用。240 h后收获细胞，并用透射电子显微镜观察。结果显示，辐照后PBMC的存活期短于未辐照的PBMC。在辐照组和未辐照组中，一些PBMC培养240 h后仍存活，但细胞体积大于淋巴细胞。通过透射电子显微镜鉴定这些细胞为单核细胞（95%）或树突状细胞（5%）。辐照后的PBMC与MKN - 28细胞共培养显示，96 h后肿瘤细胞被清除。与未辐照组相比，辐照后的PBMC具有更强的杀伤肿瘤能力。结果表明，1 Gy的γ射线辐照可提高PBMC对肿瘤细胞的杀伤作用，且1 Gy的γ射线辐照还可使体外培养的PBMC中淋巴细胞的存活期缩短，但这种辐照对PBMC中单核细胞和树突状细胞的存活期影响较小。
