Venkataraman M
Department of Medicine, Mount Sinai Hospital Medical Center, Chicago, IL 60608, USA.
Cryobiology. 1995 Dec;32(6):528-34. doi: 10.1006/cryo.1995.1055.
The effect of freezing on phytohemagglutinin-induced interferon-gamma (IFN-gamma) production by human peripheral blood mononuclear cells (PBMCs) was studied. The possible mechanisms responsible for the observed effects were also analyzed. Frozen PBMCs produced significantly larger quantities of IFN-gamma than fresh cells. Like the frozen cells, the monocyte- and natural killer cell-eliminated populations of fresh PBMCs also secreted significantly larger quantities of IFN-gamma. In contrast, the freezing process had no enhancing effect on IFN-gamma production by monocyte-depleted PBMCs. Irradiated PBMCs also secreted larger quantities of IFN-gamma. The results suggest that functional inactivation of a subset of cryosensitive suppressor monocytes is associated with an increase in IFN-gamma production by the T lymphocytes. The results provide further evidence that monocytes mediate their suppressive effect through the activation of a subset of radiosensitive, immuno-down-regulatory T cells. The ability of frozen cells to produce larger quantities of IFN-gamma should be of clinical importance. For instance, cancer patients receiving frozen PBMCs as stem cell support (after myeloablative radio/chemotherapy) should benefit from the increased IFN-gamma secretion because of its potent immunoregulatory, microbicidal, and antitumor activities.
研究了冷冻对人外周血单个核细胞（PBMC）产生植物血凝素诱导的γ干扰素（IFN-γ）的影响。还分析了导致观察到的效应的可能机制。冷冻的PBMC产生的IFN-γ量明显多于新鲜细胞。与冷冻细胞一样，新鲜PBMC中去除单核细胞和自然杀伤细胞的群体也分泌了明显更多的IFN-γ。相比之下，冷冻过程对去除单核细胞的PBMC产生IFN-γ没有增强作用。经辐照的PBMC也分泌更多的IFN-γ。结果表明，对冷冻敏感的抑制性单核细胞亚群的功能失活与T淋巴细胞产生IFN-γ的增加有关。结果进一步证明，单核细胞通过激活一部分对辐射敏感、免疫下调的T细胞来介导其抑制作用。冷冻细胞产生更多IFN-γ的能力应具有临床重要性。例如，接受冷冻PBMC作为干细胞支持（在进行清髓性放疗/化疗后）的癌症患者，由于IFN-γ具有强大的免疫调节、杀菌和抗肿瘤活性，应会从其分泌增加中受益。
