Chahud Fernando, Ramalho Leandra N Z, Ramalho Fernando S, Haddad Antonio, Roque-Barreira Maria C
Department of Pathology, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
Int J Exp Pathol. 2009 Apr;90(2):166-73. doi: 10.1111/j.1365-2613.2008.00626.x.
Neutrophil influx is essential for corneal regeneration (Gan et al. 1999). KM+, a lectin from Artocarpus integrifolia, induces neutrophil migration (Santos-de-Oliveira et al. 1994). This study aims at investigating a possible effect of KM+ on corneal regeneration in rabbits. A 6.0-mm diameter area of debridement was created on the cornea of both eyes by mechanical scraping. The experimental eyes received drops of KM+ (2.5 microg/ml) every 2 h. The control eyes received buffer. The epithelial wounded areas of the lectin-treated and untreated eyes were stained with fluorescein, photographed and measured. The animals were killed 12 h (group 1, n = 5), 24 h (group 2, n = 10) and 48 h (group 3, n = 5) after the scraping. The corneas were analysed histologically (haematoxylin and eosin and immunostaining for proliferation cell nuclear antigen, p63, vascular endothelial growth factor, c-Met and laminin). No significant differences were found at the epithelial gap between treated and control eyes in the group 1. However, the number of neutrophils in the wounded area was significantly higher in treated eyes in this group. Three control and seven treated eyes were healed completely and only rare neutrophils persisted in the corneal stroma in group 2. No morphological distinction was observed between treated and control eyes in group 3. In treated corneas of group 2, there was an increase in immunostaining of factors involved in corneal healing compared to controls. Thus, topical application of KM+ may facilitate corneal epithelial wound healing in rabbits by means of a mechanism that involves increased influx of neutrophils into the wounded area induced by the lectin.
中性粒细胞的流入对角膜再生至关重要（Gan等人，1999年）。KM +是一种来自波罗蜜的凝集素，可诱导中性粒细胞迁移（Santos - de - Oliveira等人，1994年）。本研究旨在调查KM +对兔角膜再生的可能影响。通过机械刮擦在双眼角膜上创建一个直径6.0毫米的清创区域。实验眼每2小时滴入KM +（2.5微克/毫升）。对照眼滴入缓冲液。用荧光素对凝集素处理和未处理眼的上皮损伤区域进行染色、拍照和测量。刮擦后12小时（第1组，n = 5）、24小时（第2组，n = 10）和48小时（第3组，n = 5）处死动物。对角膜进行组织学分析（苏木精和伊红染色以及增殖细胞核抗原、p63、血管内皮生长因子、c - Met和层粘连蛋白的免疫染色）。在第1组中，处理眼和对照眼的上皮间隙未发现显著差异。然而，该组处理眼中损伤区域的中性粒细胞数量显著更高。在第2组中，3只对照眼和7只处理眼完全愈合，角膜基质中仅残留极少的中性粒细胞。在第3组中，处理眼和对照眼未观察到形态学差异。在第2组的处理角膜中，与对照相比，参与角膜愈合的因子免疫染色增加。因此，局部应用KM +可能通过一种涉及凝集素诱导中性粒细胞更多地流入损伤区域的机制促进兔角膜上皮伤口愈合。
