Department of Cell and Molecular Biology and Pathogenic Bioagents, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
Department of Maternal-Infant Nursing and Public Health, Escola de Enfermagem de Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
PLoS One. 2020 Mar 24;15(3):e0230633. doi: 10.1371/journal.pone.0230633. eCollection 2020.
Mast cells are connective tissue resident cells with morphological and functional characteristics that contribute to their role in allergic and inflammatory processes, host defense and maintenance of tissue homeostasis. Mast cell activation results in the release of pro-inflammatory mediators which are largely responsible for the physiological functions of mast cells. The lectin ArtinM, extracted from Artocarpus heterophyllus (jackfruit), binds to D-manose, thus inducing degranulation of mast cells. ArtinM has several immunomodulatory properties including acceleration of wound healing, and induction of cytokine release. The aim of the present study was to investigate the role of ArtinM in the activation and proliferation of mast cells. The rat mast cell line RBL-2H3 was used throughout this study. At a low concentration (0.25μg/mL), ArtinM induced mast cell activation and the release of IL-6 without stimulating the release of pre-formed or newly formed mediators. Additionally, when the cells were activated by ArtinM protein tyrosine phosphorylation was stimulated. The low concentration of ArtinM also activated the transcription factor NFkB, but not NFAT. ArtinM also affected the cell cycle and stimulated cell proliferation. Therefore, ArtinM may have therapeutic applications by modulating immune responses due to its ability to activate mast cells and promote the release of newly synthesized mediators. Additionally, ArtinM could have beneficial effects at low concentrations without degranulating mast cells and inducing allergic reactions.
肥大细胞是结缔组织驻留细胞，具有形态和功能特征，有助于其在过敏和炎症过程、宿主防御和组织稳态维持中的作用。肥大细胞激活导致促炎介质的释放，这些介质在很大程度上负责肥大细胞的生理功能。凝集素 ArtinM 从 Artocarpus heterophyllus（菠萝蜜）中提取，与 D-甘露糖结合，从而诱导肥大细胞脱颗粒。ArtinM 具有几种免疫调节特性，包括加速伤口愈合和诱导细胞因子释放。本研究旨在探讨 ArtinM 在肥大细胞激活和增殖中的作用。本研究始终使用大鼠肥大细胞瘤系 RBL-2H3。在低浓度（0.25μg/mL）下，ArtinM 诱导肥大细胞激活和白细胞介素-6 的释放，而不刺激预先形成或新形成的介质的释放。此外，当细胞被 ArtinM 蛋白酪氨酸磷酸化激活时。ArtinM 还能激活转录因子 NFkB，但不能激活 NFAT。ArtinM 还影响细胞周期并刺激细胞增殖。因此，ArtinM 可能通过激活肥大细胞并促进新合成介质的释放来调节免疫反应而具有治疗应用。此外，ArtinM 可以在不脱颗粒肥大细胞和诱导过敏反应的低浓度下发挥有益作用。
