Kim Younghoon, Oh Sejong, Park Sungsu, Kim Sae Hun
Division of Food Bioscience and Technology, College of Life Science and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
Int J Food Microbiol. 2009 May 31;131(2-3):224-32. doi: 10.1016/j.ijfoodmicro.2009.03.002. Epub 2009 Mar 9.
Here, the gene expression profiles of EHEC O157:H7 and HT-29 during the attachment stage were investigated by using duplex whole transcriptome analysis. After the initial attachment (3 h), the gene regulation systems of both the EHEC O157:H7 and HT-29 host cells were immediately remodeled. A total of 326 genes of the HT-29 cells, which involved proteins associated with the detoxification process, stress response proteins, anti-apoptosis/inflammation proteins, immune response protein, and oxidative stress proteins, were differentially regulated by more than 2.0-fold during EHEC attachment. In contrast, when HT-29 was attached to EHEC the expression of 611 genes was induced and the expression of 384 genes was reduced by more than twofold when compared to RPMI 1640-grown EHEC (16.14% of the total hybridized genes). Among the genes that were classified according to biological function, the mRNA levels of the genes involved in stress response, oxidative stress, cell signaling and cell surface proteins were significantly altered after the attachment of EHEC O157:H7. Therefore, the results of this study provide crucial insight into the genetic networks that provide host cell protection and the strategy of EHEC O157:H7 pathogenesis in gastro-intestinal (GI) tracts.
在此，通过使用双链全转录组分析研究了肠出血性大肠杆菌O157:H7和HT-29在附着阶段的基因表达谱。在初始附着（3小时）后，肠出血性大肠杆菌O157:H7和HT-29宿主细胞的基因调控系统立即发生重塑。在肠出血性大肠杆菌附着期间，HT-29细胞共有326个基因受到差异调控，差异倍数超过2.0倍，这些基因涉及与解毒过程相关的蛋白质、应激反应蛋白、抗凋亡/炎症蛋白、免疫反应蛋白和氧化应激蛋白。相比之下，当HT-29附着于肠出血性大肠杆菌时，与在RPMI 1640培养基中生长的肠出血性大肠杆菌相比，611个基因的表达被诱导，384个基因的表达降低了两倍以上（占总杂交基因的16.14%）。在根据生物学功能分类的基因中，肠出血性大肠杆菌O157:H7附着后，参与应激反应、氧化应激、细胞信号传导和细胞表面蛋白的基因的mRNA水平发生了显著变化。因此，本研究结果为提供宿主细胞保护的遗传网络以及肠出血性大肠杆菌O157:H7在胃肠道发病机制的策略提供了关键见解。
