Stratton Thomas R, García R Edwin, Applegate Bruce M, Youngblood Jeffrey P
School of Materials Engineering, Purdue University, 701 West Stadium Avenue, West Lafayette, Indiana 47907, USA.
Biomacromolecules. 2009 May 11;10(5):1173-80. doi: 10.1021/bm801416u.
Quaternized poly(4-vinyl pyridine)-based copolymers are known to be effective against a wide range of bacteria and possess biocompatible properties. Extensive testing of a wide range of copolymers is necessary to further explore and enhance the biocidal properties. However, testing is hampered by labor-intensive bacteria testing techniques. The present paper presents a new testing method, based on bioluminescent reporter strains to enable fast evaluation of bactericidal properties. The reported method enables us to create real-time characterization of bacteria behavior with far less labor than required through traditional testing methods. A mathematical model was also developed to characterize the change in bacteria populations exposed to biocides and to enable the quantitative comparison of minimum bactericidal concentrations.
已知季铵化聚（4-乙烯基吡啶）基共聚物对多种细菌有效且具有生物相容性。有必要对多种共聚物进行广泛测试，以进一步探索和增强其杀菌性能。然而，测试受到劳动密集型细菌检测技术的阻碍。本文提出了一种基于生物发光报告菌株的新测试方法，以实现对杀菌性能的快速评估。所报道的方法使我们能够以比传统测试方法少得多的工作量对细菌行为进行实时表征。还开发了一个数学模型来表征接触杀菌剂的细菌群体的变化，并能够对最低杀菌浓度进行定量比较。
