Fjorback Anja W, Pla Patrick, Müller Heidi K, Wiborg Ove, Saudou Frédéric, Nyengaard Jens R
Stereology and Electron Microscopy Research Laboratory, HistoInformatics and MIND Center, University of Aarhus, Ole Worms Allé, Building 8, DK-8000 Aarhus C, Denmark.
Biochem Biophys Res Commun. 2009 Mar 20;380(4):724-8. doi: 10.1016/j.bbrc.2009.01.128. Epub 2009 Jan 27.
The serotonin transporter is a member of the monoamine transporter family that also includes transporters of dopamine and norepinephrine. We have used sensitized acceptor emission fluorescence resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM) to study the oligomerization of SERT in HEK-MSR-239 cells, RN46A cells and in cultured hippocampal neurons. We were able to show identical FRET efficiencies in cell lines as well as in primary cultured hippocampal neurons, demonstrating that the oligomerization is cell type independent. The results obtained with both FRET approaches are very similar and furthermore, in agreement with previous results obtained by donor bleaching FRET microscopy.
血清素转运体是单胺转运体家族的成员，该家族还包括多巴胺和去甲肾上腺素转运体。我们利用敏化受体发射荧光共振能量转移（FRET）和荧光寿命成像显微镜（FLIM）来研究血清素转运体（SERT）在HEK-MSR-239细胞、RN46A细胞和培养的海马神经元中的寡聚化。我们能够在细胞系以及原代培养的海马神经元中显示相同的FRET效率，表明寡聚化与细胞类型无关。两种FRET方法获得的结果非常相似，此外，与之前通过供体漂白FRET显微镜获得的结果一致。
