Bercovich Natalia, Levin Mariano J, Vazquez Martin P
INGEBI-CONICET, Vta. de Obligado 2490, 2P, CP 1428, 1428 Buenos Aires, Argentina.
Biochem Biophys Res Commun. 2009 Mar 20;380(4):850-5. doi: 10.1016/j.bbrc.2009.01.182. Epub 2009 Feb 5.
In trypanosomes transcription is polycistronic and individual mRNAs are generated by a trans-splicing/polyadenylation coupled reaction. We identified a divergent trypanosome FIP1-like, a factor required for mRNA 3' end formation from yeasts to human. Here we showed that it is a nuclear protein with a speckled distribution essential for trypanosome viability. A strong interaction was found between TcFIP1-like and TcCPSF30, a component of the polyadenylation complex. We determined the specific amino acids in each protein involved in the interaction. Significant differences were found between the trypanosome interaction surface and its human counterpart. Although CPSF30/FIP1 interaction is known in other organisms, this is the first report mapping the interaction surface at the amino acid level.
在锥虫中，转录是多顺反子的，单个mRNA是通过反式剪接/多聚腺苷酸化偶联反应产生的。我们鉴定出一种不同的锥虫FIP1样蛋白，它是从酵母到人类的mRNA 3'端形成所需的一个因子。在此我们表明它是一种核蛋白，具有斑点状分布，对锥虫的生存能力至关重要。在TcFIP1样蛋白和多聚腺苷酸化复合物的一个组分TcCPSF30之间发现了强烈的相互作用。我们确定了每种蛋白质中参与相互作用的特定氨基酸。在锥虫的相互作用表面与其人类对应物之间发现了显著差异。尽管CPSF30/FIP1相互作用在其他生物体中是已知的，但这是第一份在氨基酸水平上绘制相互作用表面的报告。
