Franco Luisa M, Zulliger Rahel, Wolf-Schnurrbusch Ute E K, Katagiri Yoshiaki, Kaplan Henry J, Wolf Sebastian, Enzmann Volker
Department of Ophthalmology and Visual Sciences, University of Louisville, Louisville, Kentucky, USA.
Invest Ophthalmol Vis Sci. 2009 Aug;50(8):4004-10. doi: 10.1167/iovs.08-2898. Epub 2009 Apr 1.
To correlate damage to the retinal pigment epithelium (RPE) with decreased visual function after the systemic administration of sodium iodate (NaIO(3)).
Damage was produced in mice by injection of 15, 25, or 35 mg/kg NaIO(3). Visual function was assessed with the cued water maze (WM) behavioral test and the optokinetic reflex (OKR) measurement at different times after injection. Autofluorescence in whole eye flatmounts was quantified, and hematoxylin and eosin staining of paraffin sections was performed to assess changes in the outer retina.
After 15 mg/kg NaIO(3), cued WM test results were normal, whereas OKR measurements were significantly decreased at all times. Focal RPE loss began on day 21, but no significant damage to the outer nuclear layer was observed. After 25 mg/kg NaIO(3), the cued WM test was transitionally reduced and the OKR measurement again decreased at all times. Large areas of RPE loss occurred on day 14 with a reduced outer nuclear layer on the same day. With 35 mg/kg NaIO(3), the cued WM test was reduced beginning on day 14 with complete obliteration of the OKR beginning on day 3, large areas of RPE loss on the same day, and a reduced outer nuclear layer on day 7.
Stable, patchy RPE loss was observed with a low concentration of NaIO(3). The OKR measurement showed changes in visual function earlier than the cued WM test and before histologic findings were observed.
探讨全身给予碘酸钠（NaIO₃）后视网膜色素上皮（RPE）损伤与视觉功能下降之间的相关性。
通过注射15、25或35mg/kg的NaIO₃对小鼠造成损伤。在注射后的不同时间，采用线索性水迷宫（WM）行为测试和视动性反射（OKR）测量来评估视觉功能。对全眼球平铺标本的自发荧光进行定量分析，并对石蜡切片进行苏木精和伊红染色，以评估外视网膜的变化。
给予15mg/kg NaIO₃后，线索性WM测试结果正常，而OKR测量在所有时间均显著降低。局灶性RPE损失在第21天开始出现，但未观察到外核层有明显损伤。给予25mg/kg NaIO₃后，线索性WM测试暂时降低，OKR测量在所有时间再次下降。在第14天出现大面积RPE损失，同一天外核层变薄。给予35mg/kg NaIO₃后，线索性WM测试从第14天开始降低，OKR在第3天完全消失，同一天出现大面积RPE损失，第7天外核层变薄。
低浓度的NaIO₃可导致稳定的、片状的RPE损失。OKR测量比线索性WM测试更早地显示出视觉功能的变化，且早于组织学观察结果。
