Tatemoto Y, Mori M
Department of Oral and Maxillofacial Surgery, Kochi Medical School, Japan.
Cell Mol Biol. 1991;37(3):261-77.
Immunohistochemically detectable erythropoietin-like substance(Epo) in granular convoluted tubule(GCT) cells of submandibular glands (SMG's) was examined in mice in which hemolytic anemia had been induced by phenylhydrazine (ph), and in mice subjected to hypoxia, nephrectomy, or testosterone (TP) injections. Staining for Epo was negative in GCT cells of SMG's in normal mice, while positive staining occurred in GCT cells of the anemic mice and mice subjected to hypoxia or nephrectomy. A positive Epo reaction was also revealed at the luminal borders of distal tubules, and in cells of proximal and distal tubules in the kidney, and in some hepatic and spleen cells, of mice that had received combination regimens producing anemia and hypoxia, or had been nephrectomized. Increased staining of Epo was found in GCT cells of SMG's, and in proximal and distal kidney tubules of mice given the combination treatment plus TP injections. The detection of Epo in GCT cells suggests these extrarenal cells to be sites of accumulation or biosynthesis of the protein under certain specific conditions such as hemolytic anemia and hypoxia.
研究了在经苯肼（ph）诱导产生溶血性贫血的小鼠，以及经历低氧、肾切除或注射睾酮（TP）的小鼠的颌下腺（SMG）颗粒曲管（GCT）细胞中免疫组织化学可检测到的促红细胞生成素样物质（Epo）。正常小鼠SMG的GCT细胞中Epo染色为阴性，而贫血小鼠以及经历低氧或肾切除的小鼠的GCT细胞中出现阳性染色。在接受导致贫血和低氧的联合方案或已接受肾切除的小鼠的远端小管腔缘、肾近端和远端小管细胞以及一些肝和脾细胞中也发现了阳性Epo反应。在接受联合治疗加TP注射的小鼠的SMG的GCT细胞以及肾近端和远端小管中发现Epo染色增加。在GCT细胞中检测到Epo表明，在某些特定条件下，如溶血性贫血和低氧，这些肾外细胞是该蛋白的积累或生物合成部位。
