Dedeugd Casey, Wankhede Mamta, Sorg Brian S
J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, Florida 32611, USA.
Appl Opt. 2009 Apr 1;48(10):D187-97. doi: 10.1364/ao.48.00d187.
Convective oxygen transport by microvessels depends on several parameters, including red blood cell flux and oxygen saturation. We demonstrate the use of intravital microscopy techniques to measure hemoglobin saturations, red blood cell fluxes and velocities, and microvessel cross-sectional areas in regions of microvascular networks containing multiple vessels. With these methods, data can be obtained at high spatial and temporal resolution and correlations between oxygen transport and hemodynamic parameters can be assessed. In vivo data are presented for a mouse mammary adenocarcinoma grown in a dorsal skinfold window chamber model.
微血管的对流氧输送取决于几个参数，包括红细胞通量和氧饱和度。我们展示了使用活体显微镜技术来测量包含多个血管的微血管网络区域中的血红蛋白饱和度、红细胞通量和速度以及微血管横截面积。通过这些方法，可以在高空间和时间分辨率下获得数据，并评估氧输送与血流动力学参数之间的相关性。本文给出了在背皮褶窗口室模型中生长的小鼠乳腺腺癌的体内数据。
