Wei Na, Finneran Kevin T
Environmental Engineering and Sciences, Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Biodegradation. 2009 Sep;20(5):695-707. doi: 10.1007/s10532-009-9257-z. Epub 2009 Apr 2.
Methyl tert-butyl ether (MTBE) is a prevalent groundwater contaminant. In this study, three distinct MTBE-degrading, anaerobic cultures were derived from MTBE-contaminated aquifer material: cultures NW1, NW2 and NW3. The electron acceptors used are anthraquinone-2,6-disulfonate (AQDS; NW1), sulfate (NW2) and fumarate (NW3), respectively. About 1-2 mM MTBE is consistently degraded within 20-30 days in each culture. The 16S rDNA-based amplified ribosomal DNA restriction analysis (ARDRA) was used to analyze the microbial community in each culture. Results indicate novel microorganisms (i.e. no closely related known genera or species) catalyze anaerobic MTBE biodegradation, and microbial diversity varied with different electron acceptors. Tert-butyl alcohol (TBA) accumulated to nearly stoichiometric levels, and these cultures will be critical to understanding the factors that influence TBA accumulation versus degradation. The cultures presented here are the first stable anaerobic MTBE-degrading cultures that have been characterized with respect to taxonomy.
甲基叔丁基醚（MTBE）是一种常见的地下水污染物。在本研究中，从受MTBE污染的含水层物质中获得了三种不同的降解MTBE的厌氧培养物：培养物NW1、NW2和NW3。所使用的电子受体分别是蒽醌-2,6-二磺酸盐（AQDS；NW1）、硫酸盐（NW2）和富马酸盐（NW3）。在每种培养物中，约1-2 mM的MTBE在20-30天内持续降解。基于16S rDNA的扩增核糖体DNA限制性分析（ARDRA）用于分析每种培养物中的微生物群落。结果表明，新型微生物（即没有密切相关的已知属或种）催化厌氧MTBE生物降解，并且微生物多样性因不同的电子受体而异。叔丁醇（TBA）积累到接近化学计量水平，这些培养物对于理解影响TBA积累与降解的因素至关重要。本文介绍的培养物是首批已在分类学方面进行表征的稳定厌氧MTBE降解培养物。
