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Immunohistochemistry.

作者信息

Watkins Simon

机构信息

University of Pittsburgh Medical School, Pittsburgh, Pennsylvania, USA.

出版信息

Curr Protoc Cytom. 2009 Apr;Chapter 12:Unit 12.16. doi: 10.1002/0471142956.cy1216s48.

DOI:10.1002/0471142956.cy1216s48
PMID:19340808
Abstract

Immunohistochemistry is a vastly diverse and essential method for localization of proteins in cells and tissues. This unit presents methods for labeling proteins in suspension and adherent cultures and in tissue sections, using detection methods for both fluorescence and bright-field microscopy. Choices of antibodies and detection methods are discussed, and detailed troubleshooting guidelines are provided.

摘要

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