Goldstein Marsha, Watkins Simon
Bio-Reference Laboratories, Inc., Elmwood Park, New Jersey, USA.
Curr Protoc Mol Biol. 2008 Jan;Chapter 14:Unit 14.6. doi: 10.1002/0471142727.mb1406s81.
Immunohistochemistry is a vastly diverse and essential method for localization of proteins in cells and tissues. This unit presents methods for labeling proteins in suspension and adherent cultures and in tissue sections, using detection methods for both fluorescence and bright-field microscopy. Choices of antibodies and detection methods are discussed, and detailed troubleshooting guidelines are provided.