Harmsen M C, Tolner B, Kram A, Go S J, de Haan A, Wessels J G
Department of Plant Biology, University of Groningen, The Netherlands.
Curr Genet. 1991 Jul;20(1-2):137-44. doi: 10.1007/BF00312776.
La France disease of the cultivated mushroom, Agaricus bisporus, is known to be associated with the presence of a number of dsRNA segments. The nucleotide sequences of the dsRNAs M2 (1.3 kb), M1 (1.55 kb) and L3 (2.8 kb), invariably associated with the disease, were determined. Putative coding sequences for proteins with molecular weights of 38, 40 and 87 kDa were found for M2, M1 and L3 dsRNAs, respectively. The average G + C content of these dsRNAs was 43%, close to that of A. bisporus nuclear DNA. The nucleotide sequences, as well as the amino acid sequences, appear to be unique, as no matching sequences could be found among databases. S3 dsRNA (0.39 kb), which is occasionally found in large amounts in diseased mushrooms, is an internally deleted variant of M2 dsRNA and is largely composed of the non-coding ends of that dsRNA.
栽培蘑菇双孢蘑菇的法国病已知与多个双链RNA片段的存在有关。确定了始终与该病相关的双链RNA M2（1.3 kb）、M1（1.55 kb）和L3（2.8 kb）的核苷酸序列。分别在M2、M1和L3双链RNA中发现了分子量为38、40和87 kDa的蛋白质的推定编码序列。这些双链RNA的平均G + C含量为43%，与双孢蘑菇核DNA的含量相近。核苷酸序列以及氨基酸序列似乎是独特的，因为在数据库中未发现匹配序列。S3双链RNA（0.39 kb）偶尔在患病蘑菇中大量发现，它是M2双链RNA的内部缺失变体，主要由该双链RNA的非编码末端组成。
