Romaine C P, Schlagnhaufer B
Department of Plant Pathology, Pennsylvania State University, University Park 16802, USA.
Appl Environ Microbiol. 1995 Jun;61(6):2322-5. doi: 10.1128/aem.61.6.2322-2325.1995.
Reverse transcription PCR analysis was used to investigate the involvement of two RNA-genome viruses, La France isometric virus (LIV) and mushroom bacilliform virus (MBV), in the etiology of La France disease of the cultivated mushroom Agaricus bisporus. Reverse transcription PCR amplification of sequences targeted to the genomes of LIV and MBV, with a sensitivity of detection of < 10 fg of viral RNA, showed diseased mushrooms to be either singly infected by LIV or doubly infected by LIV and MBV. Of 70 geographically diverse diseased mushroom isolates, 100% were infected by LIV, whereas almost 60% of these isolates were coinfected by MBV. Of 58 mushroom isolates determined to be free of infection by LIV, 3 were found to be infected by MBV. This represents the first documented report of the independent replication of these two viruses. Our data support the hypothesis that La France disease is associated with infection by two autonomously replicating viruses in which LIV is the primary causal agent and MBV, although possibly pathogenic and capable of modulating symptoms, is not required for pathogenesis.
采用逆转录聚合酶链反应（RT-PCR）分析，研究两种RNA基因组病毒，即拉弗朗斯等轴病毒（LIV）和蘑菇杆状病毒（MBV），是否参与栽培蘑菇双孢蘑菇拉弗朗斯病的病因。针对LIV和MBV基因组的序列进行逆转录PCR扩增，检测灵敏度低于10 fg病毒RNA，结果显示患病蘑菇要么单独感染LIV，要么同时感染LIV和MBV。在70株来自不同地理区域的患病蘑菇分离株中，100%感染了LIV，而其中近60%的分离株同时感染了MBV。在58株经检测未感染LIV的蘑菇分离株中，有3株被发现感染了MBV。这是这两种病毒独立复制的首次文献报道。我们的数据支持这样一种假设，即拉弗朗斯病与两种自主复制病毒的感染有关，其中LIV是主要病原体，MBV虽然可能具有致病性且能够调节症状，但发病机制并不需要它。
