Fan Jing Jing, Zhou Jin Lian, Li Jian Hua, Cui Sheng
State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, PR China.
Cell Biol Int. 2009 Jun;33(6):684-9. doi: 10.1016/j.cellbi.2009.03.004. Epub 2009 Mar 31.
Our previous study showed that cysteine sulfinate decarboxylase (CSD) is expressed in the testis, epididymis, and ductus deferens. However, the expression of CSD and taurine concentration in the male accessory sex glands remain unknown. Therefore, we conducted immunohistochemical analysis, semi-quantitative reverse transcription polymerase chain reaction (RT-PCR), and western blotting in order to examine CSD expression in the seminal vesicle; bulbourethral gland; and prostate gland, including the dorsolateral prostate, ventral prostate, and anterior prostate. We also analyzed the tissue taurine concentration by using high-performance liquid chromatography (HPLC). Immunohistochemical analysis revealed that CSD is expressed in the tall columnar cells of the seminal vesicle, the glandular epithelium of the bulbourethral gland, and the epithelial cells of the intermediate segments of prostate gland. The taurine concentrations in the dorsolateral prostate, ventral prostate, and anterior prostate; seminal vesicle; and bulbourethral gland in terms of micromoles per gram wet weight were 6.09+/-0.67, 6.62+/-0.43, 4.14+/-0.05, 12.55+/-1.03, and 7.50+/-0.64, respectively; these values are similar to the relative expression levels of the CSD protein. These results demonstrate that CSD is expressed in the accessory sex glands of mice, and they confirm our hypothesis that male accessory sex glands are able to synthesize taurine through the CSD pathway.
我们之前的研究表明，半胱氨酸亚磺酸脱羧酶（CSD）在睾丸、附睾和输精管中表达。然而，雄性附属性腺中CSD的表达和牛磺酸浓度仍不清楚。因此，我们进行了免疫组织化学分析、半定量逆转录聚合酶链反应（RT-PCR）和蛋白质印迹，以检测精囊、尿道球腺和前列腺（包括背外侧前列腺、腹侧前列腺和前前列腺）中CSD的表达。我们还使用高效液相色谱法（HPLC）分析了组织中的牛磺酸浓度。免疫组织化学分析显示，CSD在精囊的高柱状细胞、尿道球腺的腺上皮以及前列腺中间段的上皮细胞中表达。背外侧前列腺、腹侧前列腺和前前列腺、精囊以及尿道球腺中牛磺酸的浓度，以每克湿重微摩尔数计，分别为6.09±0.67、6.62±0.43、4.14±0.05、12.55±1.03和7.50±0.64；这些值与CSD蛋白的相对表达水平相似。这些结果表明，CSD在小鼠的附属性腺中表达，并且证实了我们的假设，即雄性附属性腺能够通过CSD途径合成牛磺酸。
