Bechinger B, Seelig J
Department of Biophysical Chemistry, University of Basel, Switzerland.
Chem Phys Lipids. 1991 May-Jun;58(1-2):1-5. doi: 10.1016/0009-3084(91)90105-k.
The influence of hydration on the orientation of the phosphocholine dipole in bilayer membranes was studied with nuclear magnetic resonance. The phosphocholine headgroup of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) was deuterated at the two methylene segments. Phosphorus-31 and deuterium nuclear magnetic resonance measurements were made as a function of hydration in the range of 10-70 wt.% H2O revealing a distinct change in the alignment of the phosphocholine headgroup. With decreasing hydration the N+ end of the phosphocholine head group dipole moves closer to the hydrocarbon layer. The conformational change induced by the loss of water molecules at the membrane surface is qualitatively similar to that observed upon addition of polyhydroxyl compounds.
利用核磁共振研究了水合作用对双层膜中磷酸胆碱偶极子取向的影响。1-棕榈酰-2-油酰-sn-甘油-3-磷酸胆碱（POPC）的磷酸胆碱头部基团在两个亚甲基片段处进行了氘代。在10-70 wt.% H₂O范围内，作为水合作用的函数进行了磷-31和氘核磁共振测量，结果显示磷酸胆碱头部基团的排列发生了明显变化。随着水合作用的降低，磷酸胆碱头部基团偶极子的N⁺端向烃层靠近。膜表面水分子损失引起的构象变化在性质上与添加多羟基化合物时观察到的变化相似。
