Department of Genetics and Biotechnology, Faculty of Agricultural Sciences, University of Aarhus, Tjele, Denmark.
Mol Biol Rep. 2010 Mar;37(3):1439-49. doi: 10.1007/s11033-009-9531-5. Epub 2009 Apr 3.
The synuclein family includes three known proteins: alpha-synuclein, beta-synuclein and gamma-synuclein. beta-Synuclein inhibits the aggregation of alpha-synuclein, a protein involved in Parkinson's disease. We have cloned and characterized the cDNA sequence for porcine beta-synuclein (SNCB) from pig cerebellum using RT-PCR. Expression analysis by quantitative RT-PCR demonstrated that SNCB transcripts were highly abundant in brain tissues. SNCB mRNA was also detected early in embryogenesis and significant increases in transcript levels were observed in several brain tissues during embryo development. Radiation hybrid mapping data indicate that the porcine SNCB maps to the q arm of chromosome 2 (2q21-22). The subcellular localization of recombinant porcine beta-synuclein was determined in three different cell types and shown to be cytoplasmic.
α-突触核蛋白、β-突触核蛋白和γ-突触核蛋白。β-突触核蛋白抑制帕金森病相关蛋白α-突触核蛋白的聚集。我们使用 RT-PCR 从猪小脑克隆并鉴定了猪β-突触核蛋白（SNCB）的 cDNA 序列。定量 RT-PCR 表达分析表明，SNCB 转录本在脑组织中高度丰富。SNCB mRNA 也在胚胎发生早期被检测到，并且在胚胎发育过程中几种脑组织中的转录本水平显著增加。辐射杂交作图数据表明，猪 SNCB 位于染色体 2 的 q 臂（2q21-22）上。在三种不同的细胞类型中测定了重组猪β-突触核蛋白的亚细胞定位，结果表明其定位于细胞质。
