Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A&F University, Shaanxi Province, People's Republic of China.
Mol Biol Rep. 2010 Mar;37(3):1671-6. doi: 10.1007/s11033-009-9858-y. Epub 2009 Oct 13.
Sirt2, a NAD(+)-dependent histone deacetylase, plays a critical role in regulating lifespan, metabolism, mitosis and adipocyte differentiation. Here two bands of the porcine Sirt2 protein were found by western blotting, so we speculated existence of Sirt2 isoforms. Next, we cloned the porcine Sirt2 gene, and also found its alternative splice variant and named the novel splicing variant Sirt2(T). The complete cDNA sequence of Sirt2(T) is 1059 bp, encoding a deduced protein of 352 amino acids which is 39 amino acids shorter at the N-terminus than Sirt2. RT-PCR revealed that the Sirt2(T) mRNA is extensively expressed in porcine tissues, and can be expressed during adipocyte differentiation. In addition, immunofluorescence and transfection demonstrated that Sirt2(T) is located in the cytoplasm and nucleus.
Sirt2 是一种依赖 NAD(+)的组蛋白去乙酰化酶，在调节寿命、代谢、有丝分裂和脂肪细胞分化方面发挥着关键作用。这里通过 Western blot 发现了两条猪 Sirt2 蛋白条带，因此我们推测存在 Sirt2 同工型。接下来，我们克隆了猪 Sirt2 基因，也发现了其选择性剪接变体，并将新的剪接变体命名为 Sirt2(T)。Sirt2(T)的完整 cDNA 序列为 1059 bp，编码一个 352 个氨基酸的推定蛋白，其 N 端比 Sirt2 短 39 个氨基酸。RT-PCR 显示 Sirt2(T)mRNA 在猪组织中广泛表达，并可在脂肪细胞分化过程中表达。此外，免疫荧光和转染实验表明 Sirt2(T)位于细胞质和细胞核中。
