头颈部细胞系创建以及用于研究和药物开发的组织采集的伦理和技术考量（第一部分）：组织采集与传代技术
Ethical and technical considerations for the creation of cell lines in the head & neck and tissue harvesting for research and drug development (Part I): techniques of tissue harvesting and propagation.
作者信息
Upile Tahwinder, Jerjes Waseem, Kafas Panagiotis, Singh Sandeep U, Sudhoff Holger, Mahil Jaspal, Sandison Ann, Hopper Colin
机构信息
Head & Neck Centre, University College London Hospital, London, UK.
出版信息
Int Arch Med. 2009 Apr 3;2(1):8. doi: 10.1186/1755-7682-2-8.
BACKGROUND
Although much has been published for the development of cell lines, these were lab based and developed for scientific technical staff.
OBJECTIVE OF REVIEW
We present a simple and successful protocol for the development of cell lines and tissue harvesting for the clinical scientist. We also discuss the ethical implications of tissue retention and present a generic consent form.
CONCLUSION
The advantages of hospital-based cell line creation are numerous. We can be more certain that cell lines are developed from the particular tissues of interest and accurate anatomical and appropriate clinico-pathological control tissues are also harvested. We can also be certain of less cell line cross contamination.
背景
尽管已经发表了许多关于细胞系开发的内容，但这些都是基于实验室的，是为科研技术人员开发的。
综述目的
我们为临床科学家提供了一种简单且成功的细胞系开发及组织采集方案。我们还讨论了组织留存的伦理问题，并提供了一份通用的知情同意书。
结论
基于医院创建细胞系有诸多优势。我们可以更确定细胞系是由感兴趣的特定组织发育而来，并且还采集了精确的解剖学和合适的临床病理对照组织。我们也能确定细胞系交叉污染较少。
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