Tran Phat L, Hammond Adrienne A, Mosley Thomas, Cortez Janette, Gray Tracy, Colmer-Hamood Jane A, Shashtri Mayank, Spallholz Julian E, Hamood Abdul N, Reid Ted W
Department of Microbiology & Immunology, Texas Tech University Health Sciences Center, Lubbock, 79430, USA.
Appl Environ Microbiol. 2009 Jun;75(11):3586-92. doi: 10.1128/AEM.02683-08. Epub 2009 Apr 3.
Among the most difficult bacterial infections encountered in treating patients are wound infections, which may occur in burn victims, patients with traumatic wounds, necrotic lesions in people with diabetes, and patients with surgical wounds. Within a wound, infecting bacteria frequently develop biofilms. Many current wound dressings are impregnated with antimicrobial agents, such as silver or antibiotics. Diffusion of the agent(s) from the dressing may damage or destroy nearby healthy tissue as well as compromise the effectiveness of the dressing. In contrast, the antimicrobial agent selenium can be covalently attached to the surfaces of a dressing, prolonging its effectiveness. We examined the effectiveness of an organoselenium coating on cellulose discs in inhibiting Pseudomonas aeruginosa and Staphylococcus aureus biofilm formation. Colony biofilm assays revealed that cellulose discs coated with organoselenium completely inhibited P. aeruginosa and S. aureus biofilm formation. Scanning electron microscopy of the cellulose discs confirmed these results. Additionally, the coating on the cellulose discs was stable and effective after a week of incubation in phosphate-buffered saline. These results demonstrate that 0.2% selenium in a coating on cellulose discs effectively inhibits bacterial attachment and biofilm formation and that, unlike other antimicrobial agents, longer periods of exposure to an aqueous environment do not compromise the effectiveness of the coating.
在治疗患者时遇到的最困难的细菌感染中，伤口感染较为常见，烧伤患者、有创伤伤口的患者、糖尿病患者的坏死性病变以及手术伤口患者都可能发生伤口感染。在伤口内部，感染细菌经常形成生物膜。许多当前的伤口敷料都浸渍有抗菌剂，如银或抗生素。抗菌剂从敷料中扩散可能会损害或破坏附近的健康组织，同时也会降低敷料的有效性。相比之下，抗菌剂硒可以共价连接到敷料表面，延长其有效性。我们研究了纤维素圆盘上的有机硒涂层在抑制铜绿假单胞菌和金黄色葡萄球菌生物膜形成方面的有效性。菌落生物膜测定表明，涂有有机硒的纤维素圆盘完全抑制了铜绿假单胞菌和金黄色葡萄球菌生物膜的形成。纤维素圆盘的扫描电子显微镜检查证实了这些结果。此外，纤维素圆盘上的涂层在磷酸盐缓冲盐水中孵育一周后仍稳定且有效。这些结果表明，纤维素圆盘涂层中0.2%的硒能有效抑制细菌附着和生物膜形成，并且与其他抗菌剂不同，长时间暴露于水环境不会降低涂层的有效性。
