Agustín María Del Rosario, Stengel Peter, Kellermeier Matthias, Tücking Katrin-Stephanie, Müller Mareike
Instituto de Ciencias Biológicas y Biomédicas del Sur, INBIOSUR (UNS-CONICET), San Juan 670, Bahía Blanca 8000, Argentina.
Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur (UNS), Bahía Blanca 8000, Argentina.
Microorganisms. 2023 Mar 30;11(4):892. doi: 10.3390/microorganisms11040892.
Biofilms are often tolerant towards routine cleaning and disinfection processes. As they can grow on fabrics in household or healthcare settings, resulting in odors and serious health problems, it is necessary to contain biofilms through eradication strategies. The current study proposes a novel test model for the growth and removal of biofilms on textiles with and the opportunistic nosocomial pathogen as model organisms. To assess the biofilm removal on fabrics, (1) a detergent-based, (2) enzyme-based, and (3) combined formulation of both detergent and enzymes (F1/2) were applied. Biofilms were analyzed microscopically (FE-SEM, SEM, 3D laser scanning- and epifluorescence microscopy), via a quartz crystal microbalance with mass dissipation monitoring (QCM-D) as well as plate counting of colonies. This study indicated that spp. form robust biofilms on woven cellulose that can be efficiently removed via F1/2, proven by a significant reduction ( < 0.001) of viable bacteria in biofilms. Moreover, microscopic analysis indicated a disruption and almost complete removal of the biofilms after F1/2 treatment. QCM-D measurements further confirmed a maximal mass dissipation change after applying F1/2. The combination strategy applying both enzymes and detergent is a promising antibiofilm approach to remove bacteria from fabrics.
生物膜通常对常规清洁和消毒过程具有耐受性。由于它们可以在家庭或医疗环境中的织物上生长，导致产生异味和严重的健康问题，因此有必要通过根除策略来控制生物膜。当前的研究提出了一种以医院机会致病菌和为模式生物，用于研究纺织品上生物膜生长和去除的新型测试模型。为了评估织物上生物膜的去除情况，应用了（1）基于洗涤剂的制剂、（2）基于酶的制剂以及（3）洗涤剂和酶的组合制剂（F1/2）。通过显微镜（场发射扫描电子显微镜、扫描电子显微镜、三维激光扫描和落射荧光显微镜）、带有质量耗散监测的石英晶体微天平（QCM-D）以及菌落平板计数对生物膜进行了分析。这项研究表明，在机织纤维素上形成了坚固的生物膜，通过F1/2可以有效地去除，生物膜中活菌的显著减少（<0.001）证明了这一点。此外，显微镜分析表明在F1/2处理后生物膜受到破坏且几乎完全被去除。QCM-D测量进一步证实了应用F1/2后最大的质量耗散变化。酶和洗涤剂联合使用的策略是一种很有前景的抗生物膜方法，可用于从织物上去除细菌。
