Pharmacology Unit, School of Dentistry, University of Buenos Aires, Buenos Aires, Argentina.
Inflamm Res. 2009 Jul;58(7):423-30. doi: 10.1007/s00011-009-0008-8. Epub 2009 Apr 4.
In the present study, we investigated the relation between the inflammatory mediators such as nitric oxide, prostaglandins, and cysteinyl-leukotrienes with mucin release and the sympathetic system in submandibular glands from rats with experimental periodontitis.
Submandibular glands from rats with experimental periodontitis.
For the first experiment, rats were treated with hydrocortisone sc, 1 mg/kg for 3 days. All other experiments were carried out in isolated submandibular glands from untreated rats. Submandibular glands were treated with cysteinyl-leukotrienes, isoproterenol, NDGA, FPL 55712, L-NMMA, Nio, Nz, AMG, indomethacin, DuP 697 and atenolol.
Nitric oxide synthase activity, prostaglandin and cysteinyl-leukotriene productions and mucin secretion were determined. The Newman-Keuls statistical test was applied after analysis of variance.
In rats with periodontitis hydrocortisone-induced a 36.6% (P < 0.05) decrease in mucin release. Only cysteinyl-leukotriene production was increased in rats with ligature (79.2%, P < 0.001). Either the inhibition of cysteinyl-leukotriene production or the block of leukotriene receptor abolished the increase in mucin secretion by 25.6% (P < 0.05) and 37% (P < 0.01), respectively, in glands from rats with ligature. On the other hand, the presence of cysteinyl-leukotrienes in the incubation medium induced mucin release from submandibular glands. Atenolol diminished by 24% (P < 0.05), the increase in cysteinyl-leukotrienes observed in rats with periodontitis. Besides, isoproterenol induced cysteinyl-leukotriene production in both groups.
In submandibular glands from rats with periodontitis, the increment in mucin release and cysteinyl-leukotrienes production are related events and both are associated with the sympathetic system.
在本研究中，我们研究了炎症介质如一氧化氮、前列腺素和半胱氨酰白三烯与实验性牙周炎大鼠下颌下腺粘蛋白释放和交感神经系统之间的关系。
实验性牙周炎大鼠的下颌下腺。
在第一个实验中，大鼠接受了 3 天的 sc 氢化可的松治疗，剂量为 1mg/kg。所有其他实验均在未处理大鼠的分离下颌下腺中进行。下颌下腺用半胱氨酰白三烯、异丙肾上腺素、NDGA、FPL 55712、L-NMMA、Nio、Nz、AMG、吲哚美辛、DuP 697 和阿替洛尔处理。
测定一氧化氮合酶活性、前列腺素和半胱氨酰白三烯的产生和粘蛋白的分泌。在方差分析后应用 Newman-Keuls 统计检验。
在牙周炎大鼠中，氢化可的松诱导粘蛋白释放减少 36.6%（P < 0.05）。只有结扎大鼠的半胱氨酰白三烯产生增加（79.2%，P < 0.001）。无论是抑制半胱氨酰白三烯的产生还是阻断白三烯受体，均可使结扎大鼠的粘蛋白分泌分别增加 25.6%（P < 0.05）和 37%（P < 0.01）。另一方面，半胱氨酰白三烯在孵育培养基中的存在诱导了下颌下腺的粘蛋白释放。阿替洛尔使牙周炎大鼠的半胱氨酰白三烯增加减少 24%（P < 0.05）。此外，异丙肾上腺素在两组大鼠中均诱导了半胱氨酰白三烯的产生。
在牙周炎大鼠的下颌下腺中，粘蛋白释放和半胱氨酰白三烯产生的增加是相关事件，两者都与交感神经系统有关。
