前列腺素E2和F2α对体外培养的大鼠下颌下腺唾液腺泡释放14C标记粘蛋白的作用。

Actions of prostaglandins E2 and F2 alpha on release of 14C-labelled mucins from rat submandibular salivary acini in vitro.

作者信息

Bradbury N A, McPherson M A

机构信息

Department of Medical Biochemistry, University of Wales College of Medicine, U.K.

出版信息

Arch Oral Biol. 1987;32(10):719-22. doi: 10.1016/0003-9969(87)90115-4.

DOI:10.1016/0003-9969(87)90115-4

PMID:3482153

Abstract

Mucin secretion was studied in vitro using well-characterized preparations of isolated acini. PGE2 significantly (p less than 0.05) increased release of [14C]-glucosamine labelled mucins at the highest concentration tested (10(-5) M), but was ineffective at lower doses (10(-9)-10(-6) M). PGF2 alpha had no effect on mucin secretion over this concentration range. PGE2 (10(-9)-10(-5) M) did not modify isoproterenol stimulated mucin secretion. The cyclooxygenase inhibitor indomethacin (10(-7)-10(-5) M) did not significantly inhibit either isoproterenol or noradrenaline stimulated mucin secretion. Thus it seems that, although PGE2 significantly increases mucin secretion at a high concentration, it is unlikely that prostaglandins play a major role in modulating beta-adrenergic stimulation of mucin secretion in rat submandibular acinar cells.

摘要

利用特性明确的分离腺泡制剂在体外研究了粘蛋白分泌。在测试的最高浓度（10⁻⁵M）下，前列腺素E2（PGE2）显著（p小于0.05）增加了[¹⁴C] - 葡糖胺标记的粘蛋白的释放，但在较低剂量（10⁻⁹ - 10⁻⁶M）时无效。在该浓度范围内，前列腺素F2α（PGF2α）对粘蛋白分泌没有影响。PGE2（10⁻⁹ - 10⁻⁵M）不改变异丙肾上腺素刺激的粘蛋白分泌。环氧化酶抑制剂吲哚美辛（10⁻⁷ - 10⁻⁵M）对异丙肾上腺素或去甲肾上腺素刺激的粘蛋白分泌均无显著抑制作用。因此，尽管PGE2在高浓度时显著增加粘蛋白分泌，但前列腺素不太可能在调节大鼠下颌下腺腺泡细胞中β - 肾上腺素能刺激的粘蛋白分泌中起主要作用。