Sankaridurg Padmaja R, Markoulli Maria, de la Jara Percy Lazon, Harmis Najat, Varghese Thomas, Willcox Mark D P, Holden Brien A
Institute for Eye Research, Level 4, Rupert Myers Building, Gate 14, Barker Street Kensington, NSW 2033, Australia.
Optom Vis Sci. 2009 Apr;86(4):312-7. doi: 10.1097/opx.0b013e318199d20c.
To determine the types of microorganisms and their frequency of isolation on the external ocular surface in children wearing soft contact lenses on a daily wear schedule.
Children aged 8 to 14 years were fitted with commercially available, soft contact lenses which were worn on a daily wear basis. The upper bulbar conjunctiva and the lower lid margins of each eye were swabbed at baseline and then at 6-monthly intervals for 2 years during lens wear. Swabs were processed, cultured and microorganisms identified using standard microbiological techniques.
At baseline, 36% of the upper bulbar conjunctivae and 53% of the lower lid margins were contaminated with predominantly gram-positive bacteria. Recovery of samples with positive growth from the upper bulbar conjunctivae decreased during lens wear and ranged from 27 to 14%. The difference was statistically significant at the 12-month visit(14%, p = 0.011). The lower lid margins generally showed similar levels of contamination as at baseline except for less contamination at the 12-month visit (33%) (p = 0.05). Staphylococcus epidermidis and Propionibacterium sp. were the predominant microorganisms isolated from both the sites with and without lens wear. Gram-negative bacteria were infrequent and when isolated, the numbers of colonies were few. Fungus was isolated in a small percent (ranging from 0 to 4.8%) of eyes at various time points.
The external ocular surface of children is contaminated with gram-positive bacteria that are resident microbiota. During lens wear, there was a trend for lesser recovery of organisms from the upper bulbar conjunctival samples,but there was no change in the type of microorganisms isolated. At all times, lower lid margins showed consistently greater colonization and is a source of potential contamination of the conjunctival surface.
确定每日佩戴软性隐形眼镜的儿童眼表微生物种类及其分离频率。
为8至14岁的儿童佩戴市售软性隐形眼镜，每日佩戴。在佩戴镜片的2年期间，于基线时以及之后每隔6个月对每只眼睛的上睑球结膜和下睑缘进行擦拭取样。使用标准微生物技术对拭子进行处理、培养并鉴定微生物。
基线时，36%的上睑球结膜和53%的下睑缘主要被革兰氏阳性菌污染。佩戴镜片期间，上睑球结膜阳性生长样本的回收率下降，范围为27%至14%。在12个月随访时差异有统计学意义（14%，p = 0.011）。下睑缘的污染水平总体上与基线时相似，但在12个月随访时污染较少（33%）（p = 0.05）。表皮葡萄球菌和丙酸杆菌是在佩戴和未佩戴镜片的部位分离出的主要微生物。革兰氏阴性菌很少见，分离出时菌落数量也很少。在不同时间点，一小部分眼睛（范围为0至4.8%）分离出真菌。
儿童眼表被作为常驻微生物群的革兰氏阳性菌污染。佩戴镜片期间，上睑球结膜样本中微生物的回收率有降低趋势，但分离出的微生物类型没有变化。在所有时间，下睑缘的定植始终更多，是结膜表面潜在污染的来源。
