Radoul Marina, Centola Fabio, Rinaldo Serena, Cutruzzolà Francesca, Pecht Israel, Goldfarb Daniella
Department of Chemical Physics, Weizmann Institute of Science, Rehovot 76100, Israel.
Inorg Chem. 2009 May 4;48(9):3913-5. doi: 10.1021/ic802355y.
W-band (95 GHz) HYSCORE and pulse ENDOR are used to characterize the nitrosyl d(1) heme complex (d(1)NO) of cd(1) nitrite reductase from Pseudomonas aeruginosa in the wild type and the Y10F mutant. The spectra and the derived (14)N hyperfine and quadrupole interactions were found to be the same for wt and Y10F. This suggests that Tyr10 does not influence the NO ligand orientation in the reduced state in solution. This study is the first application of HYSCORE at high fields and shows its potential for characterizing low gamma nuclei with large hyperfine couplings.
采用W波段（95吉赫兹）的高分辨脉冲电子自旋回波包络调制谱（HYSCORE）和脉冲电子核双共振（ENDOR）技术，对野生型和Y10F突变型铜绿假单胞菌中亚硝酸还原酶的亚硝酰基d(1)血红素复合物（d(1)NO）进行了表征。结果发现，野生型和Y10F突变型的谱图以及由此得到的(14)N超精细和四极相互作用是相同的。这表明，在溶液中还原态下，酪氨酸10（Tyr10）并不影响NO配体的取向。本研究是高场HYSCORE技术的首次应用，展示了其在表征具有大超精细耦合的低γ核方面的潜力。
