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娑罗双树微卫星的成功交叉扩增揭示了热带树木娑罗双(Shorea robusta Gaertn.)的遗传变异。

Successful cross-amplification of Shorea microsatellites reveals genetic variation in the tropical tree, Shorea robusta Gaertn.

作者信息

Pandey Madhav, Geburek Thomas

机构信息

Department of Genetics, Federal Research and Training Centre for Forests, Natural Hazards and Landscape (BFW), Hauptstrasse- 7, AT-1140 Vienna, Austria.

出版信息

Hereditas. 2009 Feb;146(1):29-32. doi: 10.1111/j.1601-5223.2009.02070.x.

DOI:10.1111/j.1601-5223.2009.02070.x
PMID:19348654
Abstract

Sal (Shorea robusta) is a tree species with very good quality timber found across tropical south Asia. Due to over-exploitation, its genetic resources are highly depleted. Here we report for the first time on molecular markers in sal. Cross-species amplification of 27 Shorea microsatellites resulted in 24 of them harboring amplicons of expected sizes. Five microsatellites were moderate to highly polymorphic (N(a)=6-19). Observed and expected heterozygosities ranged from 0.49 to 0.77 and 0.52 to 0.89, respectively. These microsatellites are found to be adaptively neutral and are useful for population genetic studies in this species.

摘要

娑罗双(Shorea robusta)是一种在热带南亚地区发现的具有优质木材的树种。由于过度开发,其遗传资源已高度枯竭。在此,我们首次报告娑罗双的分子标记。对27个娑罗双微卫星进行跨物种扩增,结果发现其中24个含有预期大小的扩增子。五个微卫星具有中度至高多态性(N(a)=6-19)。观察到的杂合度和预期杂合度分别为0.49至0.77和0.52至0.89。这些微卫星被发现是适应性中性的,可用于该物种的群体遗传学研究。

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