由反相前体电形成巨型囊泡。
Electroformation of giant vesicles from an inverse phase precursor.
作者信息
Mertins Omar, da Silveira Nádya P, Pohlmann Adriana R, Schröder André P, Marques Carlos M
机构信息
Institut Charles Sadron, UPR22, Centre National de la Recherche Scientifique, Université de Strasbourg, Strasbourg, France.
出版信息
Biophys J. 2009 Apr 8;96(7):2719-26. doi: 10.1016/j.bpj.2008.12.3928.
Abstract
We discuss a simple modification of the well-known method of giant vesicle electroformation that allows for a direct addition of water-soluble species to the phospholipid bilayers. Using this modified method, we prepare phospholipid vesicles decorated with chitosan, a water-soluble polysaccharide currently investigated for potential pharmacological applications. We find that the method allows this polysaccharide with primary amino groups on every glucose subunit to be tightly bound to the membrane, rather than simply being encapsulated.
摘要
我们讨论了一种对著名的巨型囊泡电形成方法的简单改进，该改进允许将水溶性物质直接添加到磷脂双层中。使用这种改进方法，我们制备了用壳聚糖修饰的磷脂囊泡，壳聚糖是一种目前正在研究其潜在药理应用的水溶性多糖。我们发现该方法能使这种在每个葡萄糖亚基上都带有伯氨基的多糖紧密结合在膜上，而不是简单地被包裹起来。
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