Khashram M, Huggan P, Ikram R, Chambers S, Roake J A, Lewis D R
Christchurch School of Medicine, University of Otago, New Zealand.
J Wound Care. 2009 Apr;18(4):164-7. doi: 10.12968/jowc.2009.18.4.41608.
To investigate the effect of topical negative pressure, delivered using Vacuum Assisted Closure (VAC, KCI), on the microbiology of chronic, non-infected venous leg ulcers (VLUs).
Patients receiving compression therapy for a chronic VLU were recruited into this prospective pilot study. The ulcer was swabbed and VAC was applied at 125mmHg continuous sub-atmospheric pressure on day 1 for six days. Standard methods for bacteriological sampling and measuring the wound surface area were applied at baseline and at the VAC dressing changes on days 3 and 6. Log median colony forming units (CFUs) per cm2 were used for statistical analyses. The bacterial species were identified.
Seven patients were recruited into and completed the study. The median log10 CFU/cm2 on days 1, 3 and 6 were 3.5, 4.7 and 5.1 respectively. There was a significant increase in bacterial colonisation between days 1 and 6 (p<0.02). No change was observed in the identified microbiological species during therapy with VAC.
This pilot study suggests that VAC therapy increases absolute numbers of bacteria colonising non-infected leg ulcers.
KCI supplied the VAC equipment and ARANZ the SilhouetteMobile, but both had no other influence on the study.
探讨采用负压封闭引流技术（VAC，KCI公司生产）施加的局部负压对慢性、非感染性下肢静脉溃疡（VLU）微生物学的影响。
招募接受慢性VLU压迫治疗的患者参与这项前瞻性初步研究。在第1天对溃疡进行拭子采样，并以125mmHg的持续负压施加VAC治疗，持续6天。在基线以及第3天和第6天更换VAC敷料时，采用标准的细菌学采样和测量伤口表面积的方法。每平方厘米的对数中位数菌落形成单位（CFU）用于统计分析。鉴定细菌种类。
7名患者被纳入并完成了研究。第1天、第3天和第6天每平方厘米的对数10 CFU中位数分别为3.5、4.7和5.1。第1天至第6天细菌定植显著增加（p<0.02）。在VAC治疗期间，鉴定出的微生物种类未观察到变化。
这项初步研究表明，VAC治疗会增加定植于非感染性腿部溃疡的细菌绝对数量。
KCI公司提供了VAC设备，ARANZ公司提供了SilhouetteMobile，但两者对研究均无其他影响。
