Upton Thomas G, Kashemirov Boris A, McKenna Charles E, Goodman Myron F, Prakash G K Surya, Kultyshev Roman, Batra Vinod K, Shock David D, Pedersen Lars C, Beard William A, Wilson Samuel H
Department of Chemistry, University of Southern California, Los Angeles, California 90089, USA.
Org Lett. 2009 May 7;11(9):1883-6. doi: 10.1021/ol701755k.
Alpha,beta-difluoromethylene deoxynucleoside 5'-triphosphates (dNTPs, N = A or C) are advantageously obtained via phosphorylation of corresponding dNDP analogues using catalytic ATP, PEP, nucleoside diphosphate kinase, and pyruvate kinase. DNA pol beta K(d) values for the alpha,beta-CF(2) and unmodified dNTPs, alpha,beta-NH dUTP, and the alpha,beta-CH(2) analogues of dATP and dGTP are discussed in relation to the conformations of alpha,beta-CF(2) dTTP versus alpha,beta-NH dUTP bound into the enzyme active site.
α,β-二氟亚甲基脱氧核苷5'-三磷酸(dNTP,N = A或C)可通过使用催化性ATP、磷酸烯醇丙酮酸(PEP)、核苷二磷酸激酶和丙酮酸激酶对相应的dNDP类似物进行磷酸化反应而有利地获得。本文讨论了α,β-CF₂和未修饰的dNTP、α,β-NH dUTP以及dATP和dGTP的α,β-CH₂类似物的DNA聚合酶β的K(d)值,这些值与结合到酶活性位点的α,β-CF₂ dTTP相对于α,β-NH dUTP的构象有关。