Institute of Organic Chemistry and Center for Molecular Biosciences CMBI, University of Innsbruck, 6020 Innsbruck, Austria.
Bioorg Med Chem. 2012 Apr 1;20(7):2416-8. doi: 10.1016/j.bmc.2012.01.044. Epub 2012 Feb 4.
Modified nucleoside triphosphates (NTPs) represent powerful building blocks to generate nucleic acids with novel properties by enzymatic synthesis. We have recently demonstrated the access to 2'-SeCH(3)-uridine and 2'-SeCH(3)-cytidine derivatized RNAs for applications in RNA crystallography, using the corresponding nucleoside triphosphates and distinct mutants of T7 RNA polymerase. In the present note, we introduce the chemical synthesis of the novel 2'-methylseleno-2'-deoxyadenosine and -guanosine 5'-triphosphates (2'-SeCH(3)-ATP and 2'-SeCH(3)-GTP) that represent further candidates for the enzymatic RNA synthesis with engineered RNA polymerases.
修饰核苷三磷酸（NTP）是通过酶促合成生成具有新型性质的核酸的有力构建块。我们最近通过使用相应的核苷三磷酸和 T7 RNA 聚合酶的不同突变体，展示了获得 2'-SeCH(3)-尿苷和 2'-SeCH(3)-胞苷衍生 RNA 的方法，这些 RNA 可用于 RNA 晶体学。在本说明中，我们介绍了新型 2'-甲基硒代-2'-脱氧腺苷和 -鸟苷 5'-三磷酸（2'-SeCH(3)-ATP 和 2'-SeCH(3)-GTP）的化学合成，这些化合物是用工程化 RNA 聚合酶进行酶促 RNA 合成的进一步候选物。
