De Grooth B G, Doornbos R M, Van Der Werf K O, Greve J
Department of Applied Physics, University of Twente, Enschede, The Netherlands.
Cytometry. 1991;12(5):469-72. doi: 10.1002/cyto.990120513.
We have constructed a simple device by which the optimal delay time between optical measurement of a cell and the application of the droplet charging pulse can be determined directly in a flow sorter. The device consists of a stainless steel chamber in which the sorted droplets are collected. In the collection chamber the collected droplets run through a capillary where a continuous fluorescence measurement is made. With a sample of fluorescent particles, the delay time is optimal when the measured fluorescence is maximal. The measuring volume is always filled with the last droplets sorted (about 3,000). With this device, the setting of the delay time can be done in a few seconds without the need for microscopical verification. The fluorescence in the collection chamber is excited and detected via optical fibers using about 10% of the light of the existing laser from the flow cytometer and an extra photomultiplier.
我们构建了一种简单的装置，通过该装置可以在流式细胞分选仪中直接确定细胞光学测量与液滴充电脉冲施加之间的最佳延迟时间。该装置由一个不锈钢腔室组成，分选后的液滴在其中收集。在收集腔室中，收集到的液滴流经一根毛细管，在那里进行连续的荧光测量。对于荧光颗粒样本，当测量到的荧光最大时，延迟时间是最佳的。测量体积始终充满最后分选的液滴（约3000个）。使用该装置，延迟时间的设置可以在几秒钟内完成，无需显微镜验证。收集腔室中的荧光通过光纤激发和检测，使用流式细胞仪现有激光约10%的光和一个额外的光电倍增管。
