Battye F L, Darling W, Beall J
Cytometry. 1985 Sep;6(5):492-4. doi: 10.1002/cyto.990060516.
A simple device has been developed for delivering samples into a flow cytometer. Designed with economy, simplicity, and flexibility in mind, this device, having only one moving part, can be used for sample volumes as small as 20 microliter, for virtually any form of cell sample container, and for a wide range of cell concentrations. It consists essentially of a lever-operated disc valve that allows the cell sample to be loaded into a loop of tubing and then to be injected into the cytometer nozzle under pressure from a saline source. The sampler has lifted the maximum analytical throughput of a FACS II cell sorter to better than 120 samples per hour.
已开发出一种用于将样品输送到流式细胞仪中的简单装置。该装置在设计时考虑到了经济性、简易性和灵活性，只有一个活动部件，可用于小至20微升的样品体积，适用于几乎任何形式的细胞样品容器以及各种细胞浓度。它主要由一个杠杆操作的圆盘阀组成，该阀可使细胞样品装入一段管路中，然后在来自盐溶液源的压力下注入细胞仪喷嘴。该采样器已将FACS II细胞分选仪的最大分析通量提高到每小时超过120个样品。
