Kirac Deniz, Ozden Inci, Yildirim Alper, Genç Ece
Department of Biochemistry, Faculty of Medicine, Yeditepe University, Istanbul, Turkey.
Nutr Neurosci. 2009 Apr;12(2):89-94. doi: 10.1179/147683009X423256.
The aim of the present study was to investigate whether high fat consumption changes the effects of stress on both motor activity performance, striatal and cortical dopamine and serotonin metabolites in rats. The animals were fed either with high fat or standard diet for 4 weeks. Restraint stress lasting for 15 min at +4 degrees C was applied daily to stress-exposed groups. Motor activity performance was measured weekly by using motor activity monitoring systems. At the end of the study, homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA) levels of the striatum and cerebral cortex were measured by HPLCEC. It was observed that restraint stress increased locomotor activity and high-fat diet prevented this effect. Stress and high-fat intake had an additive decreasing effect on striatal HVA levels. 5-HIAA levels, on the other hand, were lower in both high fat and high fat + stress groups compared to the stress group. These results suggest that high-fat intake differentially affected the stress response on striatal dopaminergic and serotonergic neurons in rat brain regions studied and this may be related to the effects observed in motor activity performance.
本研究的目的是调查高脂肪摄入是否会改变应激对大鼠运动活动表现、纹状体和皮质多巴胺及血清素代谢物的影响。将动物分为两组，分别给予高脂肪饮食或标准饮食，持续4周。对接受应激的组每天在4摄氏度下施加持续15分钟的束缚应激。每周使用运动活动监测系统测量运动活动表现。在研究结束时，通过高效液相色谱电化学法测量纹状体和大脑皮质中高香草酸（HVA）和5-羟吲哚乙酸（5-HIAA）的水平。观察到束缚应激增加了运动活动，而高脂肪饮食阻止了这种作用。应激和高脂肪摄入对纹状体HVA水平有累加的降低作用。另一方面，与应激组相比，高脂肪组和高脂肪+应激组的5-HIAA水平均较低。这些结果表明，高脂肪摄入对所研究的大鼠脑区纹状体多巴胺能和血清素能神经元的应激反应有不同影响，这可能与在运动活动表现中观察到的效应有关。
