Schulten Véronique, Radakovics Astrid, Hartz Christina, Mari Adriano, Vazquez-Cortes Sonia, Fernandez-Rivas Montserrat, Lauer Iris, Jahn-Schmid Beatrice, Eiwegger Thomas, Scheurer Stephan, Bohle Barbara
Department of Pathophysiology, Center for Physiology, Pathophysiology and Immunology, Medical University of Vienna, Vienna, Austria.
J Allergy Clin Immunol. 2009 Jul;124(1):100-7. doi: 10.1016/j.jaci.2009.02.010. Epub 2009 Apr 7.
Pru p 3, the nonspecific lipid transfer protein from peach, is an important plant food allergen that frequently induces systemic reactions.
We sought to analyze the allergic T-cell response to Pru p 3.
PBMCs from Italian and Spanish patients with peach allergy were stimulated with purified natural Pru p 3. Allergen-specific T-cell lines were used to identify T-cell epitopes of Pru p 3. Pru p 3-specific T-cell clones (TCCs) were analyzed for allergen-induced secretion of IL-4, IFN-gamma, and IL-10 and expression of the integrin beta7, a receptor critical for gut homing.
No difference in T-cell responses of Italian and Spanish patients was found. Among several T cell-activating regions, Pru p 3(13-27), Pru p 3(34-48), Pru p 3(43-57), and Pru p 3(61-75) were most frequently recognized in 18 Pru p 3-specific T-cell lines. The majority of 32 Pru p 3-specific TCCs belonged to the T(H)2 subset. In contrast to TCCs specific for other plant food and pollen allergens, only a limited number of Pru p 3-specific TCCs produced significant amounts of IL-10. The expression of integrin beta7 on Pru p 3-specific TCCs was comparable with that observed on peanut-specific TCCs and higher compared with that seen in different pollen-specific TCCs.
The T-cell response to Pru p 3 is dominated by T(H)2 cells presumably primed in the gut. The identification of relevant T cell-activating regions provides a basis for engineering hypoallergenic variants of Pru p 3 with less IgE binding and retained T-cell stimulatory capacity for safe immunotherapy of peach allergy.
Pru p 3是桃中的非特异性脂质转移蛋白，是一种重要的植物性食物过敏原，常引发全身反应。
我们试图分析对Pru p 3的过敏性T细胞反应。
用纯化的天然Pru p 3刺激来自意大利和西班牙桃过敏患者的外周血单核细胞（PBMC）。使用过敏原特异性T细胞系鉴定Pru p 3的T细胞表位。分析Pru p 3特异性T细胞克隆（TCC）的过敏原诱导的白细胞介素-4（IL-4）、γ干扰素（IFN-γ）和白细胞介素-10分泌以及整合素β7的表达，整合素β7是肠道归巢的关键受体。
未发现意大利和西班牙患者的T细胞反应存在差异。在几个T细胞激活区域中，Pru p 3（13 - 27）、Pru p 3（34 - 48）、Pru p 3（43 - 57）和Pru p 3（61 - 75）在18个Pru p 3特异性T细胞系中最常被识别。32个Pru p 3特异性TCC中的大多数属于T辅助2（TH2）亚群。与其他植物性食物和花粉过敏原特异性TCC不同，只有有限数量的Pru p 3特异性TCC产生大量的IL-10。Pru p 3特异性TCC上整合素β7的表达与花生特异性TCC上观察到的相当，且高于不同花粉特异性TCC上的表达。
对Pru p 3的T细胞反应以可能在肠道中致敏的TH2细胞为主导。相关T细胞激活区域的鉴定为构建低变应原性的Pru p 3变体提供了基础，该变体具有较少的免疫球蛋白E（IgE）结合且保留T细胞刺激能力，用于桃过敏的安全免疫治疗。
