Bat Erhan, van Kooten Theo G, Feijen Jan, Grijpma Dirk W
Institute for Biomedical Technology (BMTI), Department of Polymer Chemistry and Biomaterials, Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.
Biomaterials. 2009 Aug;30(22):3652-61. doi: 10.1016/j.biomaterials.2009.03.033. Epub 2009 Apr 8.
A macrophage culture model was used to investigate the erosion of gamma irradiated poly(trimethylene carbonate) (PTMC) films. When the PTMC films were incubated in the culture medium, but physically separated from the cells by a membrane, no erosion occurred. In contrast, when the J774A macrophages were directly cultured on PTMC films, they adhered to the films and were found to have eroded the polymer surface. Macrophages adhered to gamma irradiated poly(epsilon-caprolactone) (PCL) controls as well, but to a lesser extent than to the PTMC films. In this case, no signs of erosion were observed. Human skin fibroblasts cultured on PTMC and PCL films as controls also adhered to the films but did not erode the surfaces. The effect of enzymes and reactive oxygen species that can be secreted by macrophages on the erosion process was assessed using aqueous solutions of cholesterol esterase, lipoprotein lipase, esterase, potassium superoxide, and hydrogen peroxide. The PTMC films eroded in aqueous enzyme solutions as well as in aqueous superoxide solutions. Cholesterol esterase and superoxide anion radicals seem to be most involved in the macrophage-mediated erosion of PTMC. This macrophage culture model is useful in assessing the influence of macrophages on the in vivo biodegradability of polymers and in elucidating the biodegradation mechanisms involved.
采用巨噬细胞培养模型研究γ射线辐照聚碳酸三亚甲基酯（PTMC）薄膜的侵蚀情况。当PTMC薄膜在培养基中孵育，但通过膜与细胞物理分离时，未发生侵蚀。相反，当J774A巨噬细胞直接培养在PTMC薄膜上时，它们会附着在薄膜上，并且发现聚合物表面被侵蚀。巨噬细胞也会附着在γ射线辐照的聚己内酯（PCL）对照上，但程度低于PTMC薄膜。在这种情况下，未观察到侵蚀迹象。作为对照，在PTMC和PCL薄膜上培养的人皮肤成纤维细胞也会附着在薄膜上，但不会侵蚀表面。使用胆固醇酯酶、脂蛋白脂肪酶、酯酶、超氧化钾和过氧化氢的水溶液评估巨噬细胞分泌的酶和活性氧对侵蚀过程的影响。PTMC薄膜在酶水溶液和超氧化物水溶液中都会被侵蚀。胆固醇酯酶和超氧阴离子自由基似乎最参与巨噬细胞介导的PTMC侵蚀。这种巨噬细胞培养模型有助于评估巨噬细胞对聚合物体内生物降解性的影响，并阐明其中涉及的生物降解机制。
