Serrano M Concepción, Pagani Raffaella, Peña Juan, Portolés M Teresa
Department of Biochemistry and Molecular Biology I, Faculty of Chemistry, Universidad Complutense, 28040-Madrid, Spain.
Biomaterials. 2005 Oct;26(29):5827-34. doi: 10.1016/j.biomaterials.2005.02.039. Epub 2005 Apr 15.
Poly(epsilon-caprolactone) (PCL) is considered as a potential substrate for wide medical applications. In previous studies we carried out the in vitro biocompatibility assessment of PCL films using L929 mouse fibroblasts, obtaining good cell behaviour but a transitory stimulation of mitochondrial activity and cell retraction. Reactive oxygen species (ROS), mainly formed in mitochondria, can impair the function of several cellular components and produce cell oxidative stress by changing the normal red-ox status of the major cell antioxidants as glutathione. The aim of this study was to measure intracellular ROS production and glutathione content of L929 fibroblasts cultured on PCL films. Cell size, internal complexity, cell cycle and lactate dehydrogenase release were also evaluated. The films were treated with NaOH before culture to improve the cell-polymer interaction. PCL induces a transitory but significant oxidative stress in L929 fibroblasts. The treatment of PCL films with NaOH reduces this effect. PCL also induces transitory changes on cell size and complexity. Nevertheless, after 7 days in culture, cells reach control levels for all the studied parameters. Neither cell cycle nor membrane integrity appears affected by this oxidative stress respect to control cells at any culture time. These results underline the cytocompatibility of PCL films and, therefore, its potential utility as a suitable scaffold in tissue engineering.
聚己内酯（PCL）被认为是一种具有广泛医学应用潜力的基质。在先前的研究中，我们使用L929小鼠成纤维细胞对PCL薄膜进行了体外生物相容性评估，观察到细胞表现良好，但线粒体活性有短暂刺激且细胞出现回缩。活性氧（ROS）主要在线粒体中形成，它会损害多种细胞成分的功能，并通过改变主要细胞抗氧化剂如谷胱甘肽的正常氧化还原状态而产生细胞氧化应激。本研究的目的是测量在PCL薄膜上培养的L929成纤维细胞的细胞内ROS产生量和谷胱甘肽含量。还评估了细胞大小、内部复杂性、细胞周期和乳酸脱氢酶释放情况。在培养前用氢氧化钠处理薄膜以改善细胞与聚合物的相互作用。PCL在L929成纤维细胞中诱导了短暂但显著的氧化应激。用氢氧化钠处理PCL薄膜可降低这种效应。PCL还会引起细胞大小和复杂性的短暂变化。然而，培养7天后，细胞在所有研究参数上均达到对照水平。在任何培养时间，相对于对照细胞，细胞周期和膜完整性均未受这种氧化应激的影响。这些结果强调了PCL薄膜的细胞相容性，因此也凸显了其作为组织工程中合适支架的潜在用途。
