McCaffery P, Tempst P, Lara G, Dräger U C
Department of Neurobiology, Harvard Medical School Boston, MA 02115.
Development. 1991 Jul;112(3):693-702. doi: 10.1242/dev.112.3.693.
An asymmetrically distributed protein in the embryonic mouse retina was identified as an aldehyde dehydrogenase through protein microsequencing. It was characterized as a cytosolic isoform with basic isoelectric point and preference for aliphatic substrates, features that resemble those of the isoform AHD-2 which is known to oxidize retinaldehyde to retinoic acid. Immunohistochemistry with aldehyde dehydrogenase antisera showed strong labeling of the dorsal retina from the early eye vesicle stage into adulthood. In addition, optic axons originating from the dorsal retina were transiently labeled during their outgrowth phase. Whereas in the embryo the enzyme was expressed in undifferentiated cells and in neurons, in the retina of the adult mouse the asymmetrically distributed isoform was mainly expressed in Müller glia, with the number of labeled glial cells varying with retinal position.
通过蛋白质微量测序，在胚胎小鼠视网膜中鉴定出一种不对称分布的蛋白质为醛脱氢酶。它被表征为一种具有碱性等电点且偏好脂肪族底物的胞质同工型，这些特征与已知将视黄醛氧化为视黄酸的同工型AHD - 2相似。用醛脱氢酶抗血清进行的免疫组织化学显示，从早期眼泡阶段到成年期，背侧视网膜都有强烈标记。此外，起源于背侧视网膜的视神经轴突在其生长阶段被短暂标记。在胚胎中，该酶在未分化细胞和神经元中表达，而在成年小鼠视网膜中，这种不对称分布的同工型主要在穆勒胶质细胞中表达，标记的胶质细胞数量随视网膜位置而变化。
