Andresen Esben Ravn, Hamm Peter
Physikalisch-Chemisches Institut, Universitat Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland.
J Phys Chem B. 2009 May 7;113(18):6520-7. doi: 10.1021/jp810397u.
We demonstrate the extension of the principle of difference Fourier transform infrared (FTIR) spectroscopy to difference 2D-IR spectroscopy. To this end, we measure difference 2D-IR spectra of the protein bacteriorhodopsin in its early J- and K-intermediates. By comparing with the static 2D-IR spectrum of the protonated Schiff base of all-trans retinal, we demonstrate that the 2D-IR spectrum of the all-trans retinal chromophore in bacteriorhodopsin can be measured with the background from the remainder of the protein completely suppressed. We discuss several models to interpret the detailed line shape of the difference 2D-IR spectrum.
我们展示了将差示傅里叶变换红外（FTIR）光谱原理扩展到差示二维红外光谱。为此，我们测量了蛋白质细菌视紫红质早期J-和K-中间体的差示二维红外光谱。通过与全反式视黄醛质子化席夫碱的静态二维红外光谱进行比较，我们证明可以在完全抑制蛋白质其余部分背景的情况下测量细菌视紫红质中全反式视黄醛发色团的二维红外光谱。我们讨论了几种模型来解释差示二维红外光谱的详细线形。
