Amelina Hanna, Cristobal Susana
Department of Biochemistry and Biophysics, Stockholm University, Sweden.
Proteome Sci. 2009 Apr 9;7:16. doi: 10.1186/1477-5956-7-16.
This study aims to analyze sex differences in mice aging kidney. We applied a proteomic technique based on subfractionation, and liquid chromatography coupled with 2-DE. Samples from male and female CD1-Swiss outbred mice from 28 weeks, 52 weeks, and 76 weeks were analysed by 2-DE, and selected proteins were identified by matrix assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS).
This proteomic analysis detected age-related changes in protein expression in 55 protein-spots, corresponding to 22 spots in males and 33 spots in females. We found a protein expression signature (PES) of aging composed by 8 spots, common for both genders. The identified proteins indicated increases in oxidative and proteolytic proteins and decreases in glycolytic proteins, and antioxidant enzymes.
Our results provide insights into the gender differences associated to the decline of kidney function in aging. Thus, we show that proteomics can provide valuable information on age-related changes in expression levels of proteins and related modifications. This pilot study is still far from providing candidates for aging-biomarkers. However, we suggest that the analysis of these proteins could suggest mechanisms of cellular aging in kidney, and improve the kidney selection for transplantation.
本研究旨在分析衰老小鼠肾脏中的性别差异。我们应用了基于亚分级分离以及液相色谱与二维电泳联用的蛋白质组学技术。对28周、52周和76周龄的雄性和雌性CD1 - 瑞士远交系小鼠的样本进行二维电泳分析，并通过基质辅助激光解吸电离飞行时间质谱（MALDI - TOF MS）鉴定所选蛋白质。
该蛋白质组学分析在55个蛋白点中检测到与年龄相关的蛋白质表达变化，其中男性有22个点，女性有33个点。我们发现了一个由8个点组成的衰老蛋白质表达特征（PES），男女共有。鉴定出的蛋白质表明氧化和蛋白水解蛋白增加，糖酵解蛋白和抗氧化酶减少。
我们的结果为衰老过程中与肾功能下降相关的性别差异提供了见解。因此，我们表明蛋白质组学可以提供有关蛋白质表达水平与年龄相关变化及相关修饰的有价值信息。这项初步研究距离提供衰老生物标志物的候选物仍有很大差距。然而，我们建议对这些蛋白质的分析可以揭示肾脏细胞衰老的机制，并改善肾脏移植的筛选。
