利用二维差异凝胶电泳对小鼠肾小球衰老进行蛋白质组分析。
Proteomic profiling of aging in glomeruli of mice by using two-dimensional differential gel electrophoresis.
作者信息
Liu Xiaodan, Fan Qiuling, Yang Gang, Wang Lining
机构信息
Department of Nephrology, First Affiliated Hospital, China Medical University, Shenyang, Liaoning, China (mainland).
出版信息
Med Sci Monit. 2015 Feb 7;21:419-25. doi: 10.12659/MSM.892512.
BACKGROUND
Glomerular proteins were analyzed by proteomics to screen proteins participating in maturation of glomeruli before senescence and to find key proteins involved in the aging process.
MATERIAL AND METHODS
Glomeruli of C57BL/6 mice at 8 and 20 weeks were separated by kidney perfusion. Proteomic profiles of glomeruli were investigated by using two-dimensional differential gel electrophoresis and MALDI-TOF mass spectrometry.
RESULTS
We identified 22 differentially expressed proteins. Among them, 3 proteins were significantly up-regulated and 19 proteins were significantly down-regulated in mature mice. Out of these 22 proteins, 18% take part in protein transport, protein targeting, and proteolysis; 5% in glycolysis; 14% in transcription; 9% in electron transport; 9% were chaperones; and 9% were hydrolases.
CONCLUSIONS
Our results provide insights into the glomerular differentially expressed proteins correlated with renal aging. In this study we found that aging altered the expression of ATP synthase subunit beta. Further studies on this protein might help to understand the mechanism of renal aging.
背景
通过蛋白质组学分析肾小球蛋白质，以筛选参与肾小球衰老前成熟过程的蛋白质，并寻找与衰老过程相关的关键蛋白质。
材料与方法
通过肾脏灌注分离8周龄和20周龄C57BL/6小鼠的肾小球。使用二维差异凝胶电泳和基质辅助激光解吸电离飞行时间质谱法研究肾小球的蛋白质组图谱。
结果
我们鉴定出22种差异表达的蛋白质。其中，3种蛋白质在成熟小鼠中显著上调，19种蛋白质显著下调。在这22种蛋白质中，18%参与蛋白质转运、蛋白质靶向和蛋白水解；5%参与糖酵解；14%参与转录；9%参与电子传递；9%是伴侣蛋白；9%是水解酶。
结论
我们的结果为与肾脏衰老相关的肾小球差异表达蛋白质提供了见解。在本研究中，我们发现衰老改变了ATP合酶β亚基的表达。对该蛋白质的进一步研究可能有助于理解肾脏衰老的机制。
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