Qian Ya-ling, Tang Hong-fang, Wang Yan-hua, Ruan Zheng, Wu Hao, Xu Cheng-min, Zhang Xing
Zhejiang Academy of Medical Sciences, Hangzhou 310013, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2008 Aug;26(8):461-4.
To investigate suitable biomarkers for workers exposure to trimethyltin chloride (TMT-cl).
Urinary samples of 44 male workers from five TMT-cl occupational poisoning incidents were collected. Methyltin mercaptide stabilizers and waste plastics used in the incidents were also collected. The levels of TMT-cl in all the samples were determined by gas chromatography. The concentration of blood potassium for each poisonings was determined compared to control group (50 male workers of a food company), and the correlation between blood potassium and urinary TMT-cl were also determined.
TMT-cl was detected in urine of all the poisonings. The results were (0.869 +/- 0.392) microg/L (severe poisoning), (0.963 +/- 0.482) microg/L (moderate poisoning), (0.716 +/- 0.384) microg/L (mild poisoning) respectively and the difference was statistically significant (P < 0.01). But the severity of the clinical status did not seem to be closely correlated to the level of urinary TMT-cl (F = 1.88, P > 0.05). In the severe poisonings, there were no differences in urinary TMT-cl on day 4 after poisoning from day 1 (P > 0.05). In contrast, urinary TMT-cl was decreased significantly on day 4 than on day 1 in mild and moderate poisonings (P < 0.01). On day 21, levels of urinary TMT-cl of all the poisonings were higher than those of the workers exposed to TMT-cl who had no clinical status (P < 0.01). Blood potassium levels of exposed group was 77.3% which was significantly lower than normal value (P < 0.01). The concentration of blood potassium was lower than normal value (3.5 mmol/L) and was correlated with the severity of the clinical status (F = 4.45, P < 0.05). Level of urinary TMT-cl of exposed group was negatively correlated with blood potassium (r = -0.4456, P < 0.01).
Level of urinary TMT-cl can be used as exposure biomarker of TMT-cl poisoning. Blood potassium is an early biomarker of effect for TMT-cl poisoning so as to find poisoning population early.
研究适用于三甲基氯化锡（TMT-cl）暴露工人的生物标志物。
收集了来自五起TMT-cl职业中毒事件的44名男性工人的尿液样本。还收集了事件中使用的甲基锡硫醇盐稳定剂和废塑料。所有样本中的TMT-cl水平通过气相色谱法测定。将每次中毒的血钾浓度与对照组（一家食品公司的50名男性工人）进行比较，并确定血钾与尿中TMT-cl之间的相关性。
所有中毒者的尿液中均检测到TMT-cl。结果分别为（0.869±0.392）μg/L（重度中毒）、（0.963±0.482）μg/L（中度中毒）、（0.716±0.384）μg/L（轻度中毒），差异有统计学意义（P<0.01）。但临床状况的严重程度似乎与尿中TMT-cl水平无密切相关性（F = 1.88，P>0.05）。在重度中毒中，中毒后第4天尿中TMT-cl与第1天相比无差异（P>0.05）。相反，轻度和中度中毒中，中毒后第4天尿中TMT-cl比第1天显著降低（P<0.01）。在第21天，所有中毒者的尿中TMT-cl水平均高于无临床症状的TMT-cl暴露工人（P<0.01）。暴露组血钾水平为正常的77.3%，显著低于正常值（P<0.01）。血钾浓度低于正常值（3.5 mmol/L），且与临床状况的严重程度相关（F = 4.45，P<0.05）。暴露组尿中TMT-cl水平与血钾呈负相关（r = -0.4456，P<0.01）。
尿中TMT-cl水平可作为TMT-cl中毒的暴露生物标志物。血钾是TMT-cl中毒效应的早期生物标志物，以便早期发现中毒人群。
